Parasitology Research

, Volume 84, Issue 5, pp 348–353

Compared vectorial transmissibility of pure and mixed clonal genotypes of Trypanosoma cruzi in Triatoma infestans

  • A. S. Pinto
  • M. de Lana
  • B. Bastrenta
  • C. Barnabé
  • V. Quesney
  • S. Noël
  • M. Tibayrenc
ORIGINAL PAPER

DOI: 10.1007/s004360050409

Cite this article as:
Pinto, A., de Lana, M., Bastrenta, B. et al. Parasitol Res (1998) 84: 348. doi:10.1007/s004360050409

Abstract

A total of 15 mixtures involving 9 different stocks attributed to the 19/20, 32 and 39 major clonal genotypes of Trypanosoma cruzi were used to infect third-instar nymphs of Triatoma infestans via an artificial feeding device. Three biological parameters were considered: (1) the percentage of infected insects (%II), (2) the number of flagellates per insect (NFI), and (3) the percentage of trypomastigotes per insect (%DIF). Genetic characterization by both multilocus enzyme electrophoresis (MLEE) and random amplification of polymorphic DNA (RAPD) indicated that in almost all cases (87%), mixtures remained present after completion of the whole cycle in the insect vector. Two lines of comparison were performed: (1) pure clonal genotypes versus corresponding mixed clonal genotypes and (2) the␣actual behavior of mixed clonal genotypes versus the expected behavior of the theoretical mixture (i.e. the␣arithmetic mean of the results observed for each of the two clonal genotypes taken separately). Statistical analyses of the variables were made difficult because of the presence of large standard deviations. Nevertheless, in several cases, mixtures differed significantly from pure clonal genotypes, and in one case the actual mixture differed significantly from the theoretical mixture. In some cases, interaction (either potentialization or reciprocal inhibition) could be suspected.

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • A. S. Pinto
    • 1
  • M. de Lana
    • 1
  • B. Bastrenta
    • 1
  • C. Barnabé
    • 1
  • V. Quesney
    • 1
  • S. Noël
    • 1
  • M. Tibayrenc
    • 1
  1. 1.Centre d'Etudes sur le Polymorphisme des Microorganismes (CEPM), Unité Mixte de Recherche n° 9926 Centre National de la␣Recherche Scientifique (CNRS)/Institut Français de Recherche Scientifique pour le Développement en Coopération (ORSTOM), ORSTOM, BP 5045, F-34032 Montpellier, Cedex 1, France Fax: +33 4 67 41 62 99FR

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