ORIGINAL PAPER

Parasitology Research

, Volume 84, Issue 1, pp 25-30

First online:

Neurons in primary culture are less efficiently infected by Toxoplasma gondii than glial cells

  • Claudine CreuzetAffiliated withLaboratoire “Signalisation cellulaire et parasites”, UFR Cochin-Port Royal, Université René Descartes, Pavillon Gustave Roussy (6ème étage), 27 rue du, Faubourg Saint Jacques, F-75674 Paris Cedex 14, France Tel: 33 01 44 41 25 77, Fax: 33 01 44 41 25 76 e-mail: fagard@infobiogen.fr
  • , Florence RobertAffiliated withLaboratoire “Signalisation cellulaire et parasites”, UFR Cochin-Port Royal, Université René Descartes, Pavillon Gustave Roussy (6ème étage), 27 rue du, Faubourg Saint Jacques, F-75674 Paris Cedex 14, France Tel: 33 01 44 41 25 77, Fax: 33 01 44 41 25 76 e-mail: fagard@infobiogen.fr
  • , Marie Paule RoisinAffiliated withLaboratoire “Signalisation cellulaire et parasites”, UFR Cochin-Port Royal, Université René Descartes, Pavillon Gustave Roussy (6ème étage), 27 rue du, Faubourg Saint Jacques, F-75674 Paris Cedex 14, France Tel: 33 01 44 41 25 77, Fax: 33 01 44 41 25 76 e-mail: fagard@infobiogen.fr
  • , Huynh Van TanAffiliated withLaboratoire “Signalisation cellulaire et parasites”, UFR Cochin-Port Royal, Université René Descartes, Pavillon Gustave Roussy (6ème étage), 27 rue du, Faubourg Saint Jacques, F-75674 Paris Cedex 14, France Tel: 33 01 44 41 25 77, Fax: 33 01 44 41 25 76 e-mail: fagard@infobiogen.fr
  • , Cyril BenesAffiliated withLaboratoire “Signalisation cellulaire et parasites”, UFR Cochin-Port Royal, Université René Descartes, Pavillon Gustave Roussy (6ème étage), 27 rue du, Faubourg Saint Jacques, F-75674 Paris Cedex 14, France Tel: 33 01 44 41 25 77, Fax: 33 01 44 41 25 76 e-mail: fagard@infobiogen.fr
  • , Jean Dupouy-CametAffiliated withLaboratoire “Signalisation cellulaire et parasites”, UFR Cochin-Port Royal, Université René Descartes, Pavillon Gustave Roussy (6ème étage), 27 rue du, Faubourg Saint Jacques, F-75674 Paris Cedex 14, France Tel: 33 01 44 41 25 77, Fax: 33 01 44 41 25 76 e-mail: fagard@infobiogen.fr
  • , Remi FagardAffiliated withLaboratoire “Signalisation cellulaire et parasites”, UFR Cochin-Port Royal, Université René Descartes, Pavillon Gustave Roussy (6ème étage), 27 rue du, Faubourg Saint Jacques, F-75674 Paris Cedex 14, France Tel: 33 01 44 41 25 77, Fax: 33 01 44 41 25 76 e-mail: fagard@infobiogen.fr

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Abstract

Infection by Toxoplasma gondii is asymptomatic, leading to an immune response that controls the disease. In immune-compromised patients, however, quiescent cysts can reactivate, leading to toxoplasmic encephalitis. We studied the infection of cells of the central nervous system in an attempt to understand the process leading to this complication. Primary cultures of rat hippocampal glial cells and neurons were infected with the virulent strain RH and examined by immunofluorescent microscopy after fixation of cells and staining with antibodies specific to the different cell types. After 24 h of infection, glial cells were highly infected and showed active division of the parasite. Neurons, on the other hand, were much less efficiently infected than glial cells, but actual penetration of the parasites was demonstrated by confocal microscopy. Whereas glial cells contained vacuoles with several parasites, the vacuoles observed in neurons usually contained one parasite or, rarely, two, indicating that the parasites inside neurons did not undergo active division. This was corroborated by determination of the incorporation of [3H]-uracil. Little is known about the mechanism of neuronal infection by Toxoplasma. The experimental setup used in this study should help to improve our understanding of neuronal infection and bring insight into the physiopathology of toxoplasmic encephalitis.