Parasitology Research

, Volume 82, Issue 7, pp 604–611

Cell-mediated immune response and T-like cells in thymectomized Oncorhynchus mykiss (Walbaum) infected with or vaccinated against the pathogenic haemoflagellate Cryptobia salmositica Katz, 1951

  • Shujuan Feng
  • P. T. K. Woo
ORIGINAL PAPER

DOI: 10.1007/s004360050172

Cite this article as:
Feng, S. & Woo, P. Parasitol Res (1996) 82: 604. doi:10.1007/s004360050172

Abstract

 T-cell-mediated delayed-type hypersensitivity (TDTH) reaction was detected in Cryptobia salmositica-infected intact and thymectomized (2 months post-thymectomy) Oncorhynchus mykiss (Walbaum). Both groups of fish showed significant induration at the site of C. salmositica antigen injection at 8, 12 and 16 weeks post-infection. A significant difference was not observed in TDTH reactions between the thymectomized and intact (control) infected fish. The total numbers of circulating leucocytes detected in infected thymectomized fish were significantly lower than those found in infected sham-thymectomized fish. The numbers of T-like cells determined (using alpha-naphthyl acid esterase assay) in thymectomized fish (9 months post-thymectomy) were similar to those seen in intact fish prior to and at 4 weeks after vaccination with an avirulent strain of C. salmositica. At 2 weeks after challenge with the pathogen the numbers of T-like cells in intact vaccinated fish increased significantly (P < 0.01) and remained high for the duration of the study (15 weeks). However, in vaccinated thymectomized fish the numbers of T-like cells remained low after parasite challenge. These results suggest that thymectomy in adult rainbow trout did not lower T-cell-mediated delayed-type hypersensitivity; however, it reduced the numbers of circulating leucocytes and retarded the proliferation of T-like cells after antigenic stimulation.

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • Shujuan Feng
    • 1
  • P. T. K. Woo
    • 1
  1. 1.Department of Zoology, University of Guelph, Guelph, Ontario, Canada N1G 2W1CA

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