ORIGINAL PAPER

Parasitology Research

, Volume 82, Issue 3, pp 257-260

Characterization of two isolates of Trypanosoma cruzi obtained from the patient Berenice, the first human case of Chagas’ disease described by Carlos Chagas in 1909

  • M. de LanaAffiliated withDepartamento de Análises Clínicas, Escola de Farmácia, Universidade Federal de Ouro Preto, Rua Costa Sena, 171, Centro, 35400, MG, Brasil
  • , Clea A. ChiariAffiliated withDepartamento de Parasitologia, Instituto de Ciências Biológicas, C.P. 2486, Universidade Federal de Minas Gerais, 31270, Belo Horizonte, MG, Brasil
  • , Egler ChiariAffiliated withDepartamento de Parasitologia, Instituto de Ciências Biológicas, C.P. 2486, Universidade Federal de Minas Gerais, 31270, Belo Horizonte, MG, Brasil
  • , Carlos M. MorelAffiliated withDepartamento de Bioquímica e Biologia Molecular, IOC-FIOCRUZ, Rio de janeiro, RJ, Brasil
  • , Antônio M. GonçalvesAffiliated withDepartamento de Bioquímica e Biologia Molecular, IOC-FIOCRUZ, Rio de janeiro, RJ, Brasil
  • , Álvaro J. RomanhaAffiliated withLaboratório de Parasitologia Celular e Molecular, Centro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte, MG, Brasil

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Abstract

 Two isolates of Trypanosoma cruzi were obtained from the patient Berenice, the first human case of Chagas’ disease (Chagas 1909), when she was 55 and 71 years old, respectively. The isolates were characterized on the basis of their epimastigote-trypomastigote differentiation in liquid media and of the electrophoretic pattern of EcoR1 digestion products of kinetoplast DNA (k-DNA) minicircles (schizodeme) and isoenzyme patterns (zymodeme). Clear differences were found between the isolates, suggesting the occurrence of a heterogeneous population of T. cruzi in the infection of this patient.