Parasitology Research

, Volume 82, Issue 3, pp 238–241

An in vitro study on the mechanism of innate immunity in Cryptobia-resistant brook charr (Salvelinus fontinalis) against Cryptobia salmositica

  • G. M. Forward
  • P. T. K. Woo

DOI: 10.1007/s004360050102

Cite this article as:
Forward, G. & Woo, P. Parasitol Res (1996) 82: 238. doi:10.1007/s004360050102


 Fresh plasma from Cryptobia-resistant brook charr (Salvelinus fontinalis) lysed Cryptobia salmositica under in vitro conditions. However, the parasite was not lysed if the plasma was heat-inactivated at 37°C for 2 h or after the addition of ethylenediaminetetraacetic acid (EDTA) to chelate the free Mg2+ ions. Addition of Mg2+ ions to EDTA-chelated plasma restored parasite lysis. Treatment of Cryptobia-resistant plasma with either ethylenebis(oxyethylenenitrilo)tetraacetic acid, a Ca2+-chelating agent, or cobra venom factor did not reduce their lytic titres. The alternative pathway of complement activation is the mechanism of innate immunity against C. salmositica. The present study also shows that there is functional heterogeneity amongst the complement components activated via the alternative pathway within a species (brook charr) and between species (brook charr and goldfish) of fishes.

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • G. M. Forward
    • 1
  • P. T. K. Woo
    • 1
  1. 1.Department of Zoology, University of Guelph, Guelph, Ontario, Canada N1G 2W1 Fax: (519) 767-1656, E-mail: pwoo@uoguelph.caCA

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