Parasitology Research

, Volume 86, Issue 4, pp 330–334

Cloning and characterization of Entamoeba histolytica antigens recognized by human secretory IgA antibodies

  • Julio César Carrero
  • Pavel Petrossian
  • Emilio Acosta
  • María Sánchez-Zerpa
  • Librado Ortiz-Ortiz
  • Juan Pedro Laclette
SHORT COMMUNICATION

DOI: 10.1007/s004360050052

Cite this article as:
Carrero, J., Petrossian, P., Acosta, E. et al. Parasitol Res (2000) 86: 330. doi:10.1007/s004360050052

Abstract

To identify the Entamoeba histolytica antigens capable of inducing secretory IgA (sIgA) responses in humans, a cDNA library from the strain HM1:IMSS was immuno-screened with saliva from patients with intestinal amebiasis or amebic liver abscess. Clones isolated with sIgA antibodies from patients with intestinal amebiasis corresponded to the known serine-rich protein isoform, a 29 kDa cysteine-rich protein and 1-α elongation factor. Clones corresponding to enolase, cyclophilin, ribosomal protein L23a, and an Hsp70 family protein were isolated with sIgA from a patient with amebic liver abscess. A glutamic acid-rich peptide (EhGARP) positive with sIgA from a patient with amebic liver abscess was also isolated; for EhGARP, no homologs were found in the protein databases. The antigens isolated are potentially useful in the development of an oral vaccine or new diagnostic tools for amebiasis.

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Julio César Carrero
    • 1
  • Pavel Petrossian
    • 1
  • Emilio Acosta
    • 1
  • María Sánchez-Zerpa
    • 1
  • Librado Ortiz-Ortiz
    • 1
  • Juan Pedro Laclette
    • 1
  1. 1.Department of Immunology, Instituto de Investigaciones Biomédicas, UNAM, A.P. 70228, 04510 México D.F., México e-mail: laclette@servidor.unam.mx Tel.: +525-622-3860; Fax: +525-622-3369MX