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Mosquito larvicidal potential of silver nanoparticles synthesized using Chomelia asiatica (Rubiaceae) against Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus (Diptera: Culicidae)

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Abstract

Mosquitoes transmit serious human diseases, causing millions of deaths every year. Mosquito control is to enhance the health and quality of life of county residents and visitors through the reduction of mosquito populations. Mosquito control is a serious concern in developing countries like India due to the lack of general awareness, development of resistance, and socioeconomic reasons. Today, nanotechnology is a promising research domain which has a wide ranging application in vector control programs. These are nontoxic, easily available at affordable prices, biodegradable, and show broad-spectrum target-specific activities against different species of vector mosquitoes. In the present study, larvicidal activity of aqueous leaf extract and silver nanoparticles (AgNPs) synthesized using C. asiatica plant leaves against late third instar larvae of Anopheles stephensi, Aedes aegypti, and Cx. quinquefasciatus. The range of varying concentrations of synthesized AgNPs (8, 16, 24, 32, and 40 μg/mL) and aqueous leaf extract (40, 80, 120, 160, and 200 μg/mL) were tested against the larvae of An. stephensi, Ae. aegypti, and Cx. quinquefasciatus. The synthesized AgNPs from C. asiatica were highly toxic than crude leaf aqueous extract in three important vector mosquito species. The results were recorded from UV–Vis spectrum, Fourier transform infrared spectroscopy, scanning electron microscopy, and energy-dispersive X-ray spectroscopy analysis (EDX). Considerable mortality was evident after the treatment of C. asiatica for all three important vector mosquitoes. The LC50 and LC90 values of C. asiatica aqueous leaf extract appeared to be effective against An. stephensi (LC50, 90.17 μg/mL; LC90, 165.18 μg/mL) followed by Ae. aegypti (LC50, 96.59 μg/mL; LC90, 173.83 μg/mL) and Cx. quinquefasciatus (LC50, 103.08 μg/mL; LC90, 183.16 μg/mL). Synthesized AgNPs against the vector mosquitoes of An. stephensi, Ae. aegypti, and Cx. quinquefasciatus had the following LC50 and LC90 values: An. stephensi had LC50 and LC90 values of 17.95 and 33.03 μg/mL; Ae. aegypti had LC50 and LC90 values of 19.32 and 34.87 μg/mL; and Cx. quinquefasciatus had LC50 and LC90 values of 20.92 and 37.41 μg/mL. No mortality was observed in the control. These results suggest that the leaf aqueous extracts of C. asiatica and green synthesis of silver nanoparticles have the potential to be used as an ideal eco-friendly approach for the control of An. stephensi, Ae. aegypti, and Cx. quinquefasciatus. This is the first report on the mosquito larvicidal activity of the plant extracts and synthesized AgNPs.

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References

  • Abdul Rahuman A, Venkatesan P, GeethaK GG, Bagavan A, Kamaraj C (2008) Mosquito larvicidal activity of gluanol acetate, a tetracyclic triterpenes derived from Ficus racemosa Linn. Parasitol Res 103:333–339

    Article  PubMed  Google Scholar 

  • Amer A, Mehlhorn H (2006) Persistency of larvicidal effects of plant oil extracts under different storage conditions. Parasitol Res 99:473–477

    Article  PubMed  Google Scholar 

  • Amerasan D, Murugan K, Kovendan K, Mahesh Kumar P, Panneerselvam C, Subramaniam J, John William S, Hwang JS (2012) Adulticidal and repellent properties of Cassia tora Linn. (Family: Caesalpinaceae) against Culex quinquefasciatus, Aedes aegypti, and Anopheles stephensi. Parasitol Res 111(5):1953–1964

    Article  PubMed  Google Scholar 

  • Ankamwar B, Damle C, Absar A, Mural S (2005) Biosynthesis of gold and silver nanoparticles using Emblica officinalis fruit extract, their phase transfer and transmetallation in an organic solution. J Nanosci Nanotechnol 10:1665–1671

    Article  CAS  Google Scholar 

  • Arjunan NK, Murugan K, Rejeeth C, Madhiyazhagan P, Barnard DR (2012) Green synthesis of silver nanoparticles for the control of mosquito vectors of malaria, filariasis, and dengue. Vector-Borne Zoonotic Dis 12(3):262–268

    Article  PubMed  Google Scholar 

  • Bansal SK, Singh KV, Kumar S (2009) Larvicidal activity of the extracts from different parts of the plant Solanum xanthocarpum against important mosquito vectors in the arid region. J Environ Biol 30(2):221–226

    PubMed  CAS  Google Scholar 

  • Barik TK, Kamaraju R, Gowswami A (2012) Silica nanoparticles a potential new insecticide for mosquito vector control. Parasitol Res 111:1075–1083

    Article  PubMed  Google Scholar 

  • Begum NA, Mondal S, Basu S, Laskar RA, Mandal D (2009) Biogenic synthesis of Au and Ag nanoparticles using aqueous solutions of Black Tea leaf extracts. Colloids Surf B: Biointerfaces 71(1):113–118

    Article  PubMed  CAS  Google Scholar 

  • Dethloff GM, Naddy RB, Gorsuch JW (2007) Effects of sodium chloride on chronic silver toxicity to early life stages of rainbow trout (Oncorhynchus mykiss). Environ Toxicol Chem 26:1717–1725

    Article  PubMed  CAS  Google Scholar 

  • Elango G, Bagavan A, Kamaraj C, Zahir AA, Rahuman AA (2009) Oviposition-deterrent, ovicidal, and repellent activities of indigenous plant extracts against Anopheles subpictus Grassi (Diptera: Culicidae). Parasitol Res 105(6):1567–1576

    Article  PubMed  CAS  Google Scholar 

  • Elumalai EK, Prasad TN, Hemachandran J, Therasa VS, Thirumalai T, David E (2010) Extracellular synthesis of silver nanoparticles using leaves of Euphorbia hirta and their antibacterial activities. Int J Pharm Sci Res 2:549–554

    CAS  Google Scholar 

  • Finney DJ (1971) Probit analysis, vol 551. Cambridge University Press, London, pp 68–72

    Google Scholar 

  • Goodsell DS (2004) Bionanotechnology: lessons from nature. Wiley, Hoboken

    Book  Google Scholar 

  • Govindarajan M (2010a) Larvicidal efficacy of Ficus benghalensis L. plant leaf extracts against Culex quinquefasciatus Say, Aedes aegypti L. and Anopheles stephensi L. (Diptera: Culicidae). Eur Rev Med Pharmacol Sci 14(2):107–111

    PubMed  CAS  Google Scholar 

  • Govindarajan M (2010b) Larvicidal and repellent activities of Sida acuta Burm. F. (family: Malvaceae) against three important vector mosquitoes. Asian Pac J Trop Med 3(9):691–695

    Article  Google Scholar 

  • Govindarajan M (2011a) Evaluation of indigenous plant extracts against the malarial vector, Anopheles stephensi (Liston) (Diptera: Culicidae). Parasitol Res 109:93–103

    Article  PubMed  Google Scholar 

  • Govindarajan M (2011b) Larvicidal and repellent properties of some essential oils against Culex tritaeniorhynchus Giles and Anopheles subpictus Grassi (Diptera: Culicidae). Asian Pac J Trop Med 4(2):106–111

    Article  PubMed  CAS  Google Scholar 

  • Govindarajan M, Jebanesan A, Pushpanathan T (2008a) Larvicidal and ovicidal activity of Cassia fistula Linn. leaf extract against filarial and malarial vector mosquitoes. Parasitol Res 102(2):289–292

    Article  PubMed  CAS  Google Scholar 

  • Govindarajan M, Jebanesan A, Pushpanathan T, Samidurai K (2008b) Studies on effect of Acalypha indica L. (Euphorbiaceae) leaf extracts on the malarial vector, Anopheles stephensi Liston (Diptera: Culicidae). Parasitol Res 103(3):691–695

    Article  PubMed  CAS  Google Scholar 

  • Govindarajan M, Jebanesan A, Reetha D (2005) Larvicidal effect of extracellular secondary metabolites of different fungi against the mosquito, Culex quinquefasciatus Say. Trop Biomed 22(1):1–3

    PubMed  CAS  Google Scholar 

  • Govindarajan M, Sivakumar R (2012) Adulticidal and repellent properties of indigenous plant extracts against Culex quinquefasciatus and Aedes aegypti (Diptera: Culicidae). Parasitol Res 110:1607–1620

    Article  PubMed  Google Scholar 

  • Govindarajan M, Sivakumar R, Amsath A, Niraimathi S (2011) Mosquito larvicidal properties of Ficus benghalensis L. (Family: Moraceae) against Culex tritaeniorhynchus Giles and Anopheles subpictus Grassi (Diptera: Culicidae). Asian Pac J Trop Med 4(7):505–509

    Article  PubMed  CAS  Google Scholar 

  • Huang J, LiQ SD, Lu Y, Su Y, Yang X, Wang H, Wang Y, He N, Shao W, Hong J, Chen C (2007) Biosynthesis of silver and gold nanoparticles by novel sundried Cinnamomum camphora leaf. Nanotechnology 18:105104

    Article  CAS  Google Scholar 

  • Jayaseelan C, Rahuman AA, Rajakumar G, Santhoshkumar T, Kirthi AV, Marimuthu S, Bagavan A, Kamaraj C, Zahir AA, Elango G, Velayutham K, Rao KV, Karthik L, Raveendran S (2011a) Efficacy of plant-mediated synthesized silver nanoparticles against hematophagous parasites. Parasitol Res 111(2):921–933

    Article  PubMed  Google Scholar 

  • Jayaseelan C, Rahuman AA, Rajakumar G, Vishnu Kirthi A, Santhoshkumar T, Marimuthu S, Bagavan A, Kamaraj C, Zahir AA, Elango G (2011b) Synthesis of pediculocidal and larvicidal silver nanoparticles by leaf extract from heart leaf moonseed plant. Tinospora cordifolia Miers. Parasitol Res 109(1):185–194

    Article  PubMed  Google Scholar 

  • Kamaraj C, Bagavan A, Rahuman AA, Zahir AA, Elango G, Pandiyan G (2009) Larvicidal potential of medicinal plant extracts against Anopheles subpictus Grassi and Culex tritaeniorhynchus Giles (Diptera: Culicidae). Parasitol Res 104(5):1163–1171

    Article  PubMed  CAS  Google Scholar 

  • Kim KJ, SungWS SBK, Moon SK, Choi JS, Kim JG, Lee DG (2009) Antifungal activity and mode of action of silver nanoparticles on Candida albicans. Biometals 22(2):235–242

    Article  PubMed  CAS  Google Scholar 

  • Kirthi AV, Rahuman AA, Rajakumar G, Marimuthu S, Santhoshkumar T, Jayaseelan C, Velayutham K (2011) Acaricidal, pediculocidal and larvicidal activity of synthesized ZnO nanoparticles using wet chemical route against blood feeding parasites. Parasitol Res 109(2):461–472

    Article  PubMed  Google Scholar 

  • Kovendan K, Arivoli S, Maheshwaran R, Baskar K, Vincent S (2012) Larvicidal efficacy of Sphaeranthus indicus, Cleistanthus collinus and Murraya koenigii leaf extracts against filarial vector, Culex quinquefasciatus Say (Diptera: Culicidae). Parasitol Res 111(3):1025–1035

    Article  PubMed  Google Scholar 

  • Kumar V, Yadav SC, Yadav SK (2010) Syzygium cumini leaf and seed extract mediated biosynthesis of silver nanoparticles and their characterization. J Chem Technol Biotechnol 85(10):1301–1309

    Article  CAS  Google Scholar 

  • Kumar V, Yadav SK (2009) Plant-mediated synthesis of silver and gold nanoparticles and their applications. J Chem Technol Biotechnol 84:151–157

    Article  CAS  Google Scholar 

  • Lee SE (2000) Mosquito larvicidal activity of pipernonaline, a piperidine alkaloid derived from long pepper, Piper longum. J Am Mosq Control Assoc 16:245–247

    PubMed  CAS  Google Scholar 

  • Macedo ME, Consoli RA, Grandi TS, dos Anjos AM, De Oliveira AB, Mendes NM, Queiróz RO, Zani CL (1997) Screening of Asteraceae (Compositae) plant extracts for larvicidal activity against Aedes fluviatilis (Diptera: Culicidae). Mem Inst Oswaldo Cruz 92:565–570

    Article  PubMed  CAS  Google Scholar 

  • Manusadzianas L, Grigutyt R, Jurkonien S, Karitonas R, Sadauskas K, Férard JF, Cotelle S, Foucaud L (2009) Toxicity of zinc oxide nanoparticle suspensions to aquatic biota. METZ ISTA14: VIII 30–IX 04

  • Marimuthu S, Rahuman AA, Rajakumar G, Santhoshkumar T, Kirthi AV, Jayaseelan C, Bagavan A, Zahir AA, Elango G, Kamaraj C (2010) Evaluation of green synthesized silver nanoparticles against parasites. Parasitol Res 108(6):1541–1549

    Article  PubMed  Google Scholar 

  • Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramfrez JT, Yacaman MJ (2005) The bactericidal effect of silver nanoparticles. Nanotechnology 16:2346–2353

    Article  PubMed  CAS  Google Scholar 

  • Nadworny PL, Wang J, Tredget EE, Burrell RE (2008) Antiinflammatory activity of nanocrystalline silver in a porcine contact dermatitis model. Nanomedicine 4(3):241–251

    Article  PubMed  CAS  Google Scholar 

  • Panneerselvam C, Ponarulselvam S, Murugan K (2011) Potential antiplasmodial activity of synthesized silver nanoparticle using Andrographis paniculata Nees (Acanthaceae). Arch Appl Sci Res 3(6):208–217

    CAS  Google Scholar 

  • Patil CD, Borase HP, Patil SV, Salunkhe RB, Salunkhe BK (2012) Larvicidal activity of silver nanoparticles synthesized using Pergularia daemia plant latex against Aedes aegypti and Anopheles stephensi and non target fish Poicillia reticulata. Parasitol Res 111(2):555–562

    Article  PubMed  Google Scholar 

  • Ponarulselvam S, Panneerselvam C, Murugan K, Aarthi A, Kalimuthu K, Thangamani S (2012) Synthesis of silver nanoparticles using leaves of Catharanthus roseus Linn. G. Don and their antiplasmodial activities. Asian Pac J Trop Biomed 574–580

  • Priyadarshini K, Murugan K, Panneerselvam C, Ponarulselvam S, Hwang J-S, Nicoletti M (2012) Biolarvicidal and pupicidal potential of silver nanoparticles synthesized using Euphorbia hirta against Anopheles stephensi Liston (Diptera: Culicidae). Parasitol Res 111(3):997–1006

    Article  PubMed  Google Scholar 

  • Rajakumar G, Abdul Rahuman A (2011) Larvicidal activity of synthesized silver nanoparticles using Eclipta prostrata leaf extract against filariasis and malaria vectors. Acta Trop 118(3):196–203

    Article  PubMed  CAS  Google Scholar 

  • Rogers JV, Parkinson CV, Choi YW, Speshock JL, Hussain SM (2008) A preliminary assessment of silver nanoparticle inhibition of monkeypox virus plaque formation. Nanoscale Res Lett 3:129–133

    Article  PubMed Central  CAS  Google Scholar 

  • Salunkhe RB, Patil SV, Patil CD, Salunke BK (2011) Larvicidal potential of silver nanoparticles synthesized using fungus Cochliobolus lunatus against Aedes aegypti (Linnaeus, 1762) and Anopheles stephensi Liston (Diptera; Culicidae). Parasitol Res 109(3):823–831

    Article  PubMed  Google Scholar 

  • Santhoshkumar T, Rahuman AA, Rajakumar G, Marimuthu S, Bagavan A, Jayaseelan C, Zahir AA, Elango G, Kamaraj C (2011) Synthesis of silver nanoparticles using Nelumbo nucifera leaf extract and its larvicidal activity against malaria and filariasis vectors. Parasitol Res 108(3):693–702

    Article  PubMed  Google Scholar 

  • Sathishkumar M, Sneha K, Won SW, Cho CWS, Kim Yun YS (2009) Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity. Colloids SurfB: Biointerfaces 73:332–338

    Article  CAS  Google Scholar 

  • Shankar SS, Rai A, Ahmad A, Sastry MJ (2004) Rapid synthesis of Au, Ag and bimetallic Au shell nanoparticles using Neem. J Colloid Interface Sci 275:496–502

    Article  PubMed  CAS  Google Scholar 

  • Song JY, Kim BS (2009) Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess Biosyst Eng 32:79–84

    Article  PubMed  CAS  Google Scholar 

  • Soni N, Prakash S (2012) Efficacy of fungus mediated silver and gold nanoparticles against Aedes aegypti larvae. Parasitol Res 110:175–184

    Article  PubMed  Google Scholar 

  • Tian N, Liu Z, Huang J, Luo G, Liu S, Liu X (2007) Isolation and preparation of flavonoids from the leaves of Nelumbo nucifera Gaertn by preparative reversed-phase high-performance liquid chromatography. Se Pu 25:88–92

    PubMed  CAS  Google Scholar 

  • Tripathi A, Chandrasekaran N, Raichur AM, Mukherjee A (2009) Antibacterial applications of silver nanoparticles synthesized by aqueous extract of Azadirachta indica (Neem) leaves. J Biomed Nanotechnol 5(1):93–98

    Article  PubMed  CAS  Google Scholar 

  • Veerekumar K, Govindarajan M, Rajeswary M (2013) Green synthesis of silver nanoparticles using Sida acuta (Malvaceae) leaf extract against Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi (Diptera: Culicidae). Parasitol Res 112(12):4073–4085

    Article  Google Scholar 

  • Veerekumar K, Govindarajan M, Rajeswary M (2014) Low-cost and eco-friendly green synthesis of silver nanoparticles using Feronia elephantum (Rutaceae) against Culex quinquefasciatus, Anopheles stephensi, and Aedes aegypti (Diptera: Culicidae). Parasitol Res 113:1775–1785

    Article  Google Scholar 

  • Vivekanandhan S, Misra M, Mohanty AK (2009) Biological synthesis of silver nanoparticles using Glycine max (soybean) leaf extract: an investigation on different soybean varieties. J Nanosci Nanotechnol 9(12):6828–6833

    Article  PubMed  CAS  Google Scholar 

  • World Health Organization (2005) Guidelines for laboratory and field testing of mosquito larvicides. Communicable disease control, prevention and eradication, WHO pesticide evaluation scheme. WHO, Geneva, WHO/CDS/WHOPES/GCDPP/1.3

  • World Health Organization (2012a) Lymphatic filariasis. http://www.who.int/mediacentre/factsheets/fs102/en/. Accessed 10 Mar 2014

  • World Health Organization (2012b) WHO 10 facts on malaria. http://www.who.int/features/factfiles/malaria/en/index.html. Accessed 10 Mar 2014

  • World Health Organization (2012c) Dengue and severe dengue. http://www.who.int/mediacentre/factsheets/fs1117/en/. Accessed 10 Mar 2014

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Acknowledgments

The authors would like to thank Professor and Head of the Department of Zoology, Annamalai University for the laboratory facilities provided. The authors would also like to acknowledge the cooperation of staff members of the VCRC (ICMR), Pondicherry.

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  1. Unit of Vector Control, Phytochemistry and Nanotechnology, Department of Zoology, Annamalai University, Annamalainagar, 608 002, Tamil Nadu, India

    Udaiyan Muthukumaran, Marimuthu Govindarajan & Mohan Rajeswary

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  1. Udaiyan Muthukumaran
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Correspondence to Marimuthu Govindarajan.

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Muthukumaran, U., Govindarajan, M. & Rajeswary, M. Mosquito larvicidal potential of silver nanoparticles synthesized using Chomelia asiatica (Rubiaceae) against Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus (Diptera: Culicidae). Parasitol Res 114, 989–999 (2015). https://doi.org/10.1007/s00436-014-4265-2

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