Parasitology Research

, Volume 111, Issue 3, pp 1093–1101

Organ-specific testosterone-insensitive response of miRNA expression of C57BL/6 mice to Plasmodium chabaudi malaria

  • Saleh Al-Quraishy
  • Mohamed A. Dkhil
  • Denis Delic
  • Abdel Azeem Abdel-Baki
  • Frank Wunderlich
Original Paper

DOI: 10.1007/s00436-012-2937-3

Cite this article as:
Al-Quraishy, S., Dkhil, M.A., Delic, D. et al. Parasitol Res (2012) 111: 1093. doi:10.1007/s00436-012-2937-3

Abstract

Increasing evidence critically implicates miRNAs in the pathogenesis of diseases, but little is known in context with infectious diseases. This study investigates as to whether the testosterone-induced persistent susceptibility to blood-stage malaria of Plasmodium chabaudi coincides with changes in miRNA expression of the anti-malaria effectors sites spleen and liver. Female C57BL/6 mice were treated with vehicle or testosterone (T) for 3 weeks. Then, T treatment was discontinued for 12 weeks before challenge with 106P. chabaudi-parasitized erythrocytes. The miRNA expression was examined after 12 weeks of T withdrawal and during infections at peak parasitemia on day 8 p.i. using miRXplore™ microarray technology. P. chabaudi infections induce an organ-specific response of miRNA expression. We can identify 25 miRNA species to be downregulated by more than 2-fold in the spleen and 169 miRNA species in the liver. Among these 194 miRNA species, there are 12 common miRNA species that are downregulated by 0.48–0.14-fold in both spleen and liver, which are miR-194, miR-192, miR-193A-3P, miR-145, miR-16, miR-99A, miR-99B, miR-15A, miR-152, let-7G, let-7B, and miR-455-3P. Only in the liver, there is an upregulation of the miR-142-5p by 2.5-fold and miR-342-3p by 5.1-fold. After 12 weeks of T withdrawal, the spleen exhibits only the miR-200A that is upregulated by 2.7-fold. In the liver, miR-376B, miR-493*, and miR-188-3P are upregulated by 2.4-fold, 2.2-fold, and 2.1-fold, respectively, and miR-347, miR-200A, and miR-200B are downregulated by approximately 0.4-fold. Upon infection, however, these changes are not sustained, i.e., the miRNA expressions of both spleen and liver of T-pretreated mice exhibit the same response to P. chabaudi malaria as that of vehicle-treated control mice. Our data suggest (1) that the P. chabaudi-induced downregulation of miRNA expression in spleen and liver is required to allow the upregulation of their numerous target genes in response to infection, and (2) that the T-induced persistent susceptibility to P. chabaudi does not affect the responsiveness of miRNA expression in spleen and liver to blood-stage malaria.

Supplementary material

436_2012_2937_Fig4_ESM.jpg (458 kb)
Fig. S1

Expression levels of hepatic miRNA species of unknown functions downregulated by P. chabaudi malaria. Green color represents downregulation as indicated in the logarithmic color scale bar shown in Fig. 2 (JPEG 458 kb)

436_2012_2937_MOESM1_ESM.tif (550 kb)
High resolution image (TIFF 550 kb)
436_2012_2937_MOESM2_ESM.xlsx (16 kb)
Table S1Liver-specific deregulation of miRNA expression of unknown function induced by P. chabaudi malaria in vehicle-treated control mice (XLSX 16 kb)
436_2012_2937_MOESM3_ESM.xlsx (12 kb)
Table S2MicroRNA expression of spleen and liver of T-pretreated and vehicle-treated mice during P. chabaudi malaria on day 8 p.i. (XLSX 12 kb)

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Saleh Al-Quraishy
    • 1
  • Mohamed A. Dkhil
    • 1
    • 2
  • Denis Delic
    • 3
    • 5
  • Abdel Azeem Abdel-Baki
    • 1
    • 4
  • Frank Wunderlich
    • 3
  1. 1.Department of Zoology, College of ScienceKing Saud UniversityRiyadhSaudi Arabia
  2. 2.Department of Zoology and Entomology, Faculty of ScienceHelwan UniversityCairoEgypt
  3. 3.Department of Molecular ParasitologyHeinrich-Heine UniversityDuesseldorfGermany
  4. 4.Department of Zoology, Faculty of ScienceBeni-Suef UniversityCairoEgypt
  5. 5.Max Planck Institute for Neurological ResearchCologneGermany