Parasitology Research

, Volume 110, Issue 1, pp 473–477

Isolation of Acanthamoeba species in surface waters of Gilan province-north of Iran

  • Mohammad Reza Mahmoudi
  • Niloofar Taghipour
  • Mohammad Eftekhar
  • Ali Haghighi
  • Panagiotis Karanis
Short Communication

DOI: 10.1007/s00436-011-2530-1

Cite this article as:
Mahmoudi, M.R., Taghipour, N., Eftekhar, M. et al. Parasitol Res (2012) 110: 473. doi:10.1007/s00436-011-2530-1

Abstract

We analyzed water samples to determine the prevalence of free-living Acanthamoeba in water sources from Gilan, greater area, Iran. A total of 27 surface water samples were collected from environmental sources, including natural (rivers, lakes, springs, and lagoon) and freshwater source. The samples were filtrated and transferred to non-nutrient agar plates seeded with Escherichia coli and incubated for 2 to 7 days at 30°C or 42°C. The plates were examined by microscopy to morphologically identify Acanthamoeba species. Following DNA extraction, PCR was used to confirm the microscopically identification. A total of 19 out of 27 samples (70.3%) were positive for Acanthamoeba species based on the morphological criteria, and 14 (73.7%) were confirmed by PCR method. The high frequency of Acanthamoeba spp. in different environmental water sources of Gilan is an alert for the public health related to water sources in Iran.

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Mohammad Reza Mahmoudi
    • 1
    • 2
    • 3
  • Niloofar Taghipour
    • 4
  • Mohammad Eftekhar
    • 4
  • Ali Haghighi
    • 4
  • Panagiotis Karanis
    • 3
    • 5
  1. 1.Department of Parasitology and MycologySchool of MedicineTehranIran
  2. 2.Research Center of Fascioliasis and Parasitic DiseaseGuilan University of Medical SciencesRashtIran
  3. 3.Molecular and Medical ParasitologyMedical School, University of Cologne, Center of Anatomy, Institute IICologneGermany
  4. 4.Department of Parasitology and MycologySchool of Medicine, Shahid Beheshti University of Medical SciencesTehranIran
  5. 5.Medical School, University of Cologne, Center of Anatomy, Institute II, Molecular and Medical Parasitology LabCologneGermany