Parasitology Research

, 106:341

Cervine genotype is the major Cryptosporidium genotype in sheep in China

  • Yongli Wang
  • Yaoyu Feng
  • Bin Cui
  • Fuchun Jian
  • Changshen Ning
  • Rongjun Wang
  • Longxian Zhang
  • Lihua Xiao
Original Paper

DOI: 10.1007/s00436-009-1664-x

Cite this article as:
Wang, Y., Feng, Y., Cui, B. et al. Parasitol Res (2010) 106: 341. doi:10.1007/s00436-009-1664-x
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Abstract

To identify Cryptosporidium species/genotypes in sheep in China and to elucidate the endemic transmission of cryptosporidiosis, a total of 1,701 fecal samples from five farms in four prefectures in Henan Province (central China) were examined. Eighty-two Cryptosporidium-positive samples were analyzed by polymerase chain reaction (PCR)–restriction fragment length polymorphism analysis of the small subunit (SSU) rRNA gene and PCR analysis of the 60 kDa glycoprotein (gp60) gene, and 41 were further analyzed by DNA sequencing of the PCR products. The SSU rRNA-based PCR identified two Cryptosporidium species and one genotype, including the Cryptosporidium cervine genotype (74/82), Cryptosporidium andersoni (4/82), and Cryptosporidium xiaoi (4/82). The cervine genotype was found in all age groups, C. xiaoi in lambs, and C. andersoni in ewes. There were intragenetic differences in the SSU rRNA gene sequences of the Cryptosporidium cervine genotype and C. xiaoi. No Cryptosporidium parvum was detected by both SSU rRNA- and gp60-based PCR assays. These findings suggest that sheep are a potential source for zoonotic infections of the Cryptosporidium cervine genotype.

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Yongli Wang
    • 1
  • Yaoyu Feng
    • 2
  • Bin Cui
    • 1
  • Fuchun Jian
    • 1
  • Changshen Ning
    • 1
  • Rongjun Wang
    • 1
  • Longxian Zhang
    • 1
  • Lihua Xiao
    • 3
  1. 1.The College of Animal Science and Veterinary MedicineHenan Agricultural UniversityZhengzhouPeople’s Republic of China
  2. 2.School of Resource and Environmental EngineeringEast China University of Science and TechnologyShanghaiPeople’s Republic of China
  3. 3.Division of Parasitic Diseases, National Center for Zoonotic, Vector-Borne and Enteric Diseases, Centers for Disease Control and PreventionU.S. Department of Health and Human ServicesAtlantaUSA

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