Parasitology Research

, 105:169

Saline extract of Taenia saginata metacestodes as an alternative antigen for the immunodiagnosis of neurocysticercosis in human cerebrospinal fluid

  • Heliana B. Oliveira
  • Gleyce A. Machado
  • Maria do Rosário F. Gonçalves-Pires
  • Leandro P. Moura
  • Julia M. Costa-Cruz
Original Paper

DOI: 10.1007/s00436-009-1379-z

Cite this article as:
Oliveira, H.B., Machado, G.A., Gonçalves-Pires, M..R.F. et al. Parasitol Res (2009) 105: 169. doi:10.1007/s00436-009-1379-z

Abstract

The aim of the present research was to test the application of Taenia saginata metacestodes as an alternative antigen for use in enzyme-linked immunosorbent assay (ELISA) and Western Blotting (WB) tests compared with the use of metacestodes antigen of Taenia solium in cerebrospinal fluid (CSF) samples. The samples were obtained from 35 patients with definitive neurocysticercosis (NCC)—group 1—and 44 patients with other neurological disorders (control)—group 2. The sensitivity and specificity of ELISA, using antigen obtained from T. solium, applied to the patients of group 1 yielded results of 100%. When the tests were conducted using T. saginata metacestodes, results were 100% and 93.2%, respectively. The 47–52-, 64–68-, and 70-kDa antigens showed high frequencies in CSF samples from patients with NCC when WB was conducted with both antigens. The results indicate that T. saginata metacestodes can be used as an alternative antigen for the diagnosis of human NCC in CSF samples.

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Heliana B. Oliveira
    • 1
    • 2
  • Gleyce A. Machado
    • 1
    • 2
  • Maria do Rosário F. Gonçalves-Pires
    • 1
  • Leandro P. Moura
    • 3
  • Julia M. Costa-Cruz
    • 1
  1. 1.Departamento de Imunologia, Microbiologia e Parasitologia, Instituto de Ciências BiomédicasUniversidade Federal de UberlândiaUberlândiaBrazil
  2. 2.Departamento de Ciências BiológicasUniversidade Federal de GoiásCatalãoBrazil
  3. 3.Departamento de Clínica Médica, Faculdade de MedicinaUniversidade Federal de UberlândiaUberlândiaBrazil