Parasitology Research

, Volume 104, Issue 5, pp 1053–1059

Effects of essential oils from Cymbopogon citratus (DC) Stapf., Lippia sidoides Cham., and Ocimum gratissimum L. on growth and ultrastructure of Leishmania chagasi promastigotes

  • Valéria C. S. Oliveira
  • Daniela M. S. Moura
  • José A. D. Lopes
  • Paulo P. de Andrade
  • Nicácio H. da Silva
  • Regina C. B. Q. Figueiredo
Original Paper

DOI: 10.1007/s00436-008-1288-6

Cite this article as:
Oliveira, V.C.S., Moura, D.M.S., Lopes, J.A.D. et al. Parasitol Res (2009) 104: 1053. doi:10.1007/s00436-008-1288-6
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Abstract

The current therapy for leishmaniasis, which affects annually about 2 million people, is far from satisfactory. All available drugs require parenteral administration and are potentially toxic. Plant essential oils have been traditionally used in folk medicine and appear as valuable alternative source for chemotherapeutic compounds. In this study, we demonstrated the effect of essential oils from Cymbopogon citratus, Lippia sidoides, and Ocimum gratissimum on growth and ultrastructure of Leishmania chagasi promastigote forms. Steam distillation was used to isolate the essential oils, and their constituents were characterized by gas chromatography coupled to mass spectrometry and nuclear magnetic resonance. All essential oils showed in vitro inhibitory action on L. chagasi promastigotes growth in a dose-dependent way, with IC50/72 h of 45, 89, and 75 μg/mL for C. citratus, L. sidoides, and O. gratissimum, respectively. Drastic morphological alterations were observed in all essential oil-treated parasites, including cell swelling, accumulation of lipid droplets in the cytoplasm, and increase of acidocalcisome volume. Furthermore, aberrant-shaped cells with multi-septate body were observed by scanning electron microscopy, suggesting an additional effect on cytokinesis. Taken together, our data show that these essential oils affect the parasite viability being the C. citratus essential oil the most effective against L. chagasi.

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Valéria C. S. Oliveira
    • 1
  • Daniela M. S. Moura
    • 2
  • José A. D. Lopes
    • 1
  • Paulo P. de Andrade
    • 3
  • Nicácio H. da Silva
    • 4
  • Regina C. B. Q. Figueiredo
    • 2
  1. 1.Departamento de Química/CCNUniversidade Federal do PiauíTeresinaBrazil
  2. 2.Departamento de MicrobiologiaCentro de Pesquisas Aggeu Magalhães/FIOCRUZRecifeBrazil
  3. 3.Departamento de GenéticaUniversidade Federal de PernambucoRecifeBrazil
  4. 4.Departamento de BioquímicaUniversidade Federal de PernambucoRecifeBrazil

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