Parasitology Research

, 103:787

Identification and characterization of a mitochondrial iron–superoxide dismutase of Cryptosporidium parvum

  • Jung-Mi Kang
  • Hyeng-Il Cheun
  • Juri Kim
  • Sung-Ung Moon
  • Soon-Jung Park
  • Tong-Soo Kim
  • Woon-Mok Sohn
  • Byoung-Kuk Na
Original Paper

DOI: 10.1007/s00436-008-1041-1

Cite this article as:
Kang, JM., Cheun, HI., Kim, J. et al. Parasitol Res (2008) 103: 787. doi:10.1007/s00436-008-1041-1
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Abstract

Cryptosporidium parvum is an intracellular protozoan parasite that causes cryptosporidiosis in mammals. In this study, we identified a gene encoding mitochondrial iron–superoxide dismutase of C. parvum (Cp-mtSOD) and characterized biochemical properties of the recombinant protein. Multiple sequence alignment of the deduced amino acid sequence of Cp-mtSOD with those of previously reported iron-containing SODs (Fe-SODs) from other protozoan parasites showed that Cp-mtSOD shares common metal-binding residues and motifs that were conserved in Fe-SODs. However, the N-terminal 26-amino acid residues of Cp-mtSOD did not show sequence identities to any other Fe-SOD sequences. Further analysis of the N-terminal presequence of Cp-mtSOD suggested that it shares common physiochemical characteristics found in mitochondria targeting sequences and predicted localization of Cp-mtSOD in the mitochondria. The recombinant Cp-mtSOD showed typical biochemical properties with other characterized Fe-SODs, including molecular structure, broad pH optimum, and sensitivity to hydrogen peroxide.

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Jung-Mi Kang
    • 1
  • Hyeng-Il Cheun
    • 2
  • Juri Kim
    • 3
  • Sung-Ung Moon
    • 2
  • Soon-Jung Park
    • 3
  • Tong-Soo Kim
    • 4
  • Woon-Mok Sohn
    • 1
  • Byoung-Kuk Na
    • 1
  1. 1.Department of Parasitology and Institute of Health SciencesGyeongsang National University College of MedicineJinjuSouth Korea
  2. 2.Department of Malaria and Parasitic DiseasesNational Institute of Health, Centers for Disease Control and PreventionSeoulSouth Korea
  3. 3.Department of Environmental Medical Biology and Institute of Tropical MedicineYonsei University College of MedicineSeoulSouth Korea
  4. 4.Department of Parasitology and Inha Research Institute for Medical SciencesInha University College of MedicineIncheonSouth Korea

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