Parasitology Research

, Volume 101, Issue 6, pp 1621–1625

Clinical and histologic evaluations of experimental Acanthamoeba keratitis

  • Zubeyde Akin Polat
  • Semra Ozcelik
  • Ayse Vural
  • Esin Yıldız
  • Ali Cetin
Original Paper

DOI: 10.1007/s00436-007-0704-7

Cite this article as:
Polat, Z.A., Ozcelik, S., Vural, A. et al. Parasitol Res (2007) 101: 1621. doi:10.1007/s00436-007-0704-7


Amoebic keratitis, a sight-threatening, progressive corneal disease, is commonly caused by ubiquitous, pathogenic, free-living Acanthamoeba spp., which are widely distributed in the environment. We investigated clinical findings and histology of Acanthamoeba keratitis in a rat cornea model. Experimental Acanthamoeba keratitis was induced in Wistar rats by intrastromal inoculation of Acanthamoeba castellanii trophozoites. The clinic features of Acanthamoeba keratitis by day 70 are observed. All rats inoculated with Acanthamoeba developed keratitis. Histologically, the eyes displayed blood vessels, edema, and amoebae in stroma. A mixed cellular response, including neutrophils, mononuclear cells, and spindle-shaped cells, was seen. In conclusion, progressive, suppurative Acanthamoeba keratitis can be induced in the rat cornea model. This rat cornea model assists researchers who study the pathogenesis of Acanthamoeba keratitis and devise treatment for this difficult condition.

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Zubeyde Akin Polat
    • 1
  • Semra Ozcelik
    • 2
  • Ayse Vural
    • 3
  • Esin Yıldız
    • 4
  • Ali Cetin
    • 5
  1. 1.CUTFAM Research CenterCumhuriyet University School of MedicineSivasTurkey
  2. 2.Department of ParasitologyCumhuriyet University School of MedicineSivasTurkey
  3. 3.Department of OphthalmologyCumhuriyet University School of MedicineSivasTurkey
  4. 4.Department of PathologyCumhuriyet University School of MedicineSivasTurkey
  5. 5.Department of Obstetrics and GynecologyCumhuriyet University School of MedicineSivasTurkey

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