Parasitology Research

, Volume 101, Issue 3, pp 703–708

Evaluation of human IgG class and subclass antibodies to a 24 kDa antigenic component of Gnathostoma spinigerum for the serodiagnosis of gnathostomiasis

Authors

  • Porntip Laummaunwai
    • Department of Parasitology, Faculty of MedicineKhon Kaen University
  • Kittisak Sawanyawisuth
    • Department of Medicine, Faculty of MedicineKhon Kaen University
  • Pewpan M. Intapan
    • Department of Parasitology, Faculty of MedicineKhon Kaen University
  • Verajit Chotmongkol
    • Department of Medicine, Faculty of MedicineKhon Kaen University
  • Chaisiri Wongkham
    • Department of Biochemistry, Faculty of MedicineKhon Kaen University
    • Department of Parasitology, Faculty of MedicineKhon Kaen University
Original Paper

DOI: 10.1007/s00436-007-0538-3

Cite this article as:
Laummaunwai, P., Sawanyawisuth, K., Intapan, P.M. et al. Parasitol Res (2007) 101: 703. doi:10.1007/s00436-007-0538-3

Abstract

The immunoglobulin G class (total IgG) and subclass (IgG1, IgG2, IgG3, and IgG4) antibody responses to the spirurid worm Gnathostoma spinigerum were analyzed by immunoblotting technique for the antibodies’ potential use in the serodiagnosis of human gnathostomiasis. Serum samples from patients with proven gnathostomiasis and from clinically suspected cases of gnathostomiasis with migratory swelling were tested. Sera from patients with other parasitic illnesses and from healthy volunteers were also analyzed. The total IgG antibody to an antigenic band of approximately 24 kDa from a somatic extract of G. spinigerum advanced third-stage larvae (GS24) gave the highest sensitivity (91.6%) and 87.8% specificity. Individual IgG subclass detection had a lower sensitivity than the detection of specific total IgG antibody, but IgG4 had a slightly higher specificity (93.9%). However, for cost effectiveness, we suggest that anti GS24 total IgG is sufficient for the routine serodiagnosis of human gnathostomiasis as well as for seroepidemiological studies in developing countries.

Copyright information

© Springer-Verlag 2007