Parasitology Research

, Volume 101, Issue 3, pp 517–525

Evaluation of recombinant HP6-Tsag, an 18 kDa Taenia saginata oncospheral adhesion protein, for the diagnosis of cysticercosis

  • Elizabeth Ferrer
  • Luís Miguel González
  • José Ángel Martínez-Escribano
  • María Eugenia González-Barderas
  • María Milagros Cortéz
  • Iris Dávila
  • Leslie J. S. Harrison
  • R. Michael E. Parkhouse
  • Teresa Gárate
Original Paper

DOI: 10.1007/s00436-007-0507-x

Cite this article as:
Ferrer, E., González, L.M., Martínez-Escribano, J.Á. et al. Parasitol Res (2007) 101: 517. doi:10.1007/s00436-007-0507-x

Abstract

With the objective of providing inexpensive and reproducible assays for the detection of antibodies indicating exposure to Taenia saginata and Taenia solium, we have evaluated the diagnostic utility of the T. saginata oncosphere adhesion protein (HP6-Tsag), expressed in baculovirus (HP6-Bac) and bacteria (HP6-GST [glutathione S-transferase]), employing enzyme-linked immunosorbent assays (ELISAs) and sera from T. saginata infected cattle, T. solium infected pigs and serum and cerebrospinal fluid (CSF) samples from clinically defined T. solium neurocysticercosis (NCC) patients. The two recombinant proteins were antigenic in all three systems, with the signal to background ratio of the HP6-Bac ELISA slightly higher than that for the HP6-GST ELISA. Assay performance in cattle was similar to previously described peptide-based ELISA assays, although NCC sample sensitivity/specificity was marginally better. The sensitivity of the HP6-Bac and HP6-GST ELISAs was close for active human NCC (77.4 and 80.6% for serum and 76.9 and 73.1% for CSF samples, respectively). In inactive human NCC, however, the sensitivity of the HP6-Bac ELISA was almost twice that of the HP6-GST ELISA. Because peptides are relatively expensive and recombinant proteins are simple and economical to produce, the latter may provide useful reagents for antibody detection in countries with endemic cysticercosis/NCC.

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Elizabeth Ferrer
    • 1
    • 2
    • 3
  • Luís Miguel González
    • 1
  • José Ángel Martínez-Escribano
    • 4
  • María Eugenia González-Barderas
    • 4
    • 8
  • María Milagros Cortéz
    • 3
  • Iris Dávila
    • 5
  • Leslie J. S. Harrison
    • 6
  • R. Michael E. Parkhouse
    • 7
  • Teresa Gárate
    • 1
  1. 1.Instituto de Salud Carlos IIICentro Nacional de MicrobiologíaMajadahonda, MadridSpain
  2. 2.Departamento de ParasitologíaUniversidad de Carabobo Sede AraguaMaracayVenezuela
  3. 3.Instituto de Investigaciones Biomédicas (BIOMED)Universidad de CaraboboMaracayVenezuela
  4. 4.Departamento de Mejora Genética y BiotecnologíaInstituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)MadridSpain
  5. 5.Departamento de ParasitologíaUniversidad de CaraboboValenciaVenezuela
  6. 6.Division of Veterinary Clinical Sciences, Centre for Tropical Veterinary Medicine, Royal (Dick) School of Veterinary Studies The University of Edinburgh, Easter Bush Veterinary CentreMidlothianUK
  7. 7.Parkhouse Instituto Gulbenkian de CiénciaOeirasPortugal
  8. 8.Fundación Jiménez DíazAvda. Reyes CatólicosMadridSpain

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