Parasitology Research

, Volume 100, Issue 4, pp 899–904

A metalloproteinase gene from the pathogenic piscine hemoflagellate, Cryptobia salmositica

  • Palmy R. Jesudhasan
  • Chung-Wei Tan
  • Patrick T. K. Woo
Short Communication

DOI: 10.1007/s00436-006-0340-7

Cite this article as:
Jesudhasan, P.R., Tan, CW. & Woo, P.T.K. Parasitol Res (2007) 100: 899. doi:10.1007/s00436-006-0340-7
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Abstract

We report the identification of a Cryptobia genomic DNA gene predicted to encode a hydrophobic protein containing a zinc metalloproteinase motif, HEXXH, and hence named it a major surface proteinase 1-like (MSP-1). The MSP-1 gene was identified using universal genome walking. Southern blot analysis revealed it to be a multicopy gene. Fragments of DNA encoding a segment homologous to the HEXXH motif of MSP-1 are widely found in bacteria, yeast, parasitic protozoans, plants, and animals including humans. These results suggest that the MSP-1 may have highly conserved functions, such as in intracellular proteolysis.

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Palmy R. Jesudhasan
    • 1
    • 2
  • Chung-Wei Tan
    • 1
  • Patrick T. K. Woo
    • 1
  1. 1.Department of Integrative BiologyUniversity of GuelphGuelphCanada
  2. 2.Department of Poultry Science, 420 Kleberg CenterTexas A & M UniversityCollege StationUSA

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