Short Communication

Parasitology Research

, Volume 100, Issue 4, pp 887-892

First online:

Cofactor-independent phosphoglycerate mutase is an essential gene in procyclic form Trypanosoma brucei

  • Appolinaire DjikengAffiliated withThe Institute for Genomic Research (TIGR) Email author 
  • , Sylvine RaverdyAffiliated withNew England Biolabs (NEB)
  • , Jeremy FosterAffiliated withNew England Biolabs (NEB)
  • , Daniella BartholomeuAffiliated withThe Institute for Genomic Research (TIGR)
  • , Yinhua ZhangAffiliated withNew England Biolabs (NEB)
  • , Najib M. El-SayedAffiliated withThe Institute for Genomic Research (TIGR)Department of Microbiology and Tropical Medicine, George Washington University
  • , Clotilde CarlowAffiliated withNew England Biolabs (NEB)

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access


Glycolysis and gluconeogenesis are, in part, driven by the interconversion of 3- and 2-phosphoglycerate (3-PG and 2-PG) which is performed by phosphoglycerate mutases (PGAMs) which can be cofactor dependant (dPGAM) or cofactor independent (iPGAM). The African trypanosome, Trypanosoma brucei, possesses the iPGAM form which is thought to play an important role in glycolysis. Here, we report on the use of RNA interference to down-regulate the T. brucei iPGAM in procyclic form T. brucei and evaluation of the resulting phenotype. We first demonstrated biochemically that depletion of the steady state levels of iPGM mRNA correlates with a marked reduction of enzyme activity. We further show that iPGAM is required for cell growth in procyclic T. brucei.