Parasitology Research

, Volume 100, Issue 4, pp 887–892

Cofactor-independent phosphoglycerate mutase is an essential gene in procyclic form Trypanosoma brucei

  • Appolinaire Djikeng
  • Sylvine Raverdy
  • Jeremy Foster
  • Daniella Bartholomeu
  • Yinhua Zhang
  • Najib M. El-Sayed
  • Clotilde Carlow
Short Communication

DOI: 10.1007/s00436-006-0332-7

Cite this article as:
Djikeng, A., Raverdy, S., Foster, J. et al. Parasitol Res (2007) 100: 887. doi:10.1007/s00436-006-0332-7

Abstract

Glycolysis and gluconeogenesis are, in part, driven by the interconversion of 3- and 2-phosphoglycerate (3-PG and 2-PG) which is performed by phosphoglycerate mutases (PGAMs) which can be cofactor dependant (dPGAM) or cofactor independent (iPGAM). The African trypanosome, Trypanosoma brucei, possesses the iPGAM form which is thought to play an important role in glycolysis. Here, we report on the use of RNA interference to down-regulate the T. brucei iPGAM in procyclic form T. brucei and evaluation of the resulting phenotype. We first demonstrated biochemically that depletion of the steady state levels of iPGM mRNA correlates with a marked reduction of enzyme activity. We further show that iPGAM is required for cell growth in procyclic T. brucei.

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Appolinaire Djikeng
    • 1
  • Sylvine Raverdy
    • 2
  • Jeremy Foster
    • 2
  • Daniella Bartholomeu
    • 1
  • Yinhua Zhang
    • 2
  • Najib M. El-Sayed
    • 1
    • 3
  • Clotilde Carlow
    • 2
  1. 1.The Institute for Genomic Research (TIGR)RockvilleUSA
  2. 2.New England Biolabs (NEB)IpswichUSA
  3. 3.Department of Microbiology and Tropical MedicineGeorge Washington UniversityWashingtonUSA