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Molecular cloning and characterization of a major egg antigen in Paragonimus westermani and its use in ELISA for the immunodiagnosis of paragonimiasis

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Abstract

A recombinant protein of a Paragonimus westermani egg antigen was produced and tested as an antigen for the serologic diagnosis of P. westermani infection. The P. westermani egg antigen gene contains a single open reading frame of 966 base pairs encoding 322 amino acids from 5′ methionine to the 3′ stop codon. The predicted amino acid sequence of this egg antigen was 40, 38, and was 35% identical to heat shock proteins from Schistosoma japonicum, Schistosoma mansoni, and Taenia saginata. The distribution this antigen was investigated in adult worms by indirect immunofluorescence assay, and found to be distributed in eggs and uteri. The specificity and sensitivity of the recombinant antigen were assessed by enzyme-linked immunosorbent assay (ELISA) using sera from patients infected with different parasites, which included 41 patients with paragonimiasis, and negative controls. The diagnostic positive and negative predictive absorbance value was 0.24 and the sensitivity of ELISA using the recombinant antigen was 90.2%, and its specificity 100%. Our results suggest that the developed recombinant major egg antigen-based ELISA offers a highly sensitive and specific assay for the diagnosis of paragonimiasis.

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References

  • Cho SY, Hong ST, Rho YH, Choi SY, Han YC (1981) Application of micro-ELISA in serodiagnosis of human paragonimiasis. Korean J Parasitol 19:151–156

    Google Scholar 

  • Cho SY, Kong Y, Yun DH, Kang SY, Kim LS, Chung YB, Yang HJ (2000) Persisting antibody reaction in paragonimiasis after praziquantel treatment is elicited mainly by egg antigens. Korean J Parasitol 38:75–84

    PubMed  CAS  Google Scholar 

  • Chung CB, Kong Y, Cho SY, Kang SY, Choi BC, Lee SH (1993) Characterization of a peroxidase excretory-secretory product of adult Paragonimus westermani. Korean J Parasitol 31:259–267

    PubMed  CAS  Google Scholar 

  • Ichikawa H, Ikeda T (1995) Excretory/secretory antigens of adult Paragonimus ohirai recognized by a monoclonal antibody. Int J Parasitol 25:725–730

    Article  PubMed  CAS  Google Scholar 

  • Ikeda T (1998) Cystatin capture enzyme-linked immunosorbent assay for immunodiagnosis of human paragonimiasis and fascioliasis. Am J Trop Med Hyg 59:286–290

    PubMed  CAS  Google Scholar 

  • Ikeda T, Oikawa Y, Nishiyama T (1996) Enzyme-linked immunosorbent assay using cysteine proteinase antigens for immunodiagnosis of human paragonimiasis. Am J Trop Med Hyg 55:434–437

    Google Scholar 

  • Imai J (1987) Evaluation of ELISA for the diagnosis of Paragonimus westermani. Trans R Soc Trop Med Hyg 81:3–6

    Article  PubMed  CAS  Google Scholar 

  • Kim TY, Joo IJ, Kang SY, Cho SY, Kong Y, Gan XX, Sukomtason K, Hong SJ (2002) Recombinant Paragonimus westermani yolk ferritin is a useful serodiagnostic antigen. J Infect Dis 185:1373–1375

    Article  PubMed  CAS  Google Scholar 

  • Knobloch J (1984) Application of different Paragonimus antigens to immunodiagnosis of human lung fluke infection. Arzneimittelforschung 34:1208–1210

    PubMed  CAS  Google Scholar 

  • Kong Y, Chung JY, Yun DH, Kim LS, Kang SH, Akira ITO, Cho SY (1997) Variation of antigenic proteins of eggs and developmental stages of Paragonimus westermani. Korean J Parasitol 35:197–202

    PubMed  CAS  Google Scholar 

  • Kong Y, Ito A, Yang HJ, Chung YB, Kasuya S, Kobayashi M, Liu YH, Cho SY (1998) Immunoglobulin G (IgG) subclass and IgE responses in human paragonimiasis caused by three different species. Clin Diagn Lab Immunol 5:474–478

    PubMed  CAS  Google Scholar 

  • Maleewong W (1997) Recent advances in diagnosis of paragonimiasis. Southeast Asian J Trop Med Public Health 28(Suppl 1):134–138

    PubMed  Google Scholar 

  • Yong TS, Seo JH, Yeo IS (1993) Serodiagnosis of human paragonimiasis by ELISA-inhibition test using monoclonal antibodies. Korean J Parasitol 31:141–147

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Parasitology and Institute of Tropical Medicine, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, 120-752, South Korea

    Jae Sik Lee, Jongweon Lee & Tai-Soon Yong

  2. Department of Family Medicine, Kwandong University College of Medicine, Goyang, 412-270, South Korea

    Sun Hyun Kim

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  1. Jae Sik Lee
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  2. Jongweon Lee
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  3. Sun Hyun Kim
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  4. Tai-Soon Yong
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Correspondence to Tai-Soon Yong.

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Lee, J.S., Lee, J., Kim, S.H. et al. Molecular cloning and characterization of a major egg antigen in Paragonimus westermani and its use in ELISA for the immunodiagnosis of paragonimiasis. Parasitol Res 100, 677–681 (2007). https://doi.org/10.1007/s00436-006-0324-7

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  • DOI: https://doi.org/10.1007/s00436-006-0324-7

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