Parasitology Research

, Volume 97, Issue 5, pp 424–428

Comparison of different PCR protocols for the detection and diagnosis of Plasmodium falciparum

  • N. Oster
  • I. Z. Abdel-Aziz
  • A. Stich
  • B. Coulibaly
  • B. Kouyatè
  • K. T. Andrews
  • J. E. McLean
  • M. Lanzer
Short Communication

DOI: 10.1007/s00436-005-1479-3

Cite this article as:
Oster, N., Abdel-Aziz, I.Z., Stich, A. et al. Parasitol Res (2005) 97: 424. doi:10.1007/s00436-005-1479-3
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Abstract

An assessment of differing PCR protocols for the diagnosis of Plasmodium falciparum infection was performed on samples from an area of holoendemic malaria transmission in western Burkina Faso. The PCR protocols had generally high sensitivities (>92%) and specificities (>69%), but the negative predictive values (NPV) were moderate and differed widely among the PCR protocols tested. These PCR protocols that amplified either the P. falciparum pfcrt gene or the small subunit ribosomal DNA were the most reliable diagnostic tools. However, the moderate NPV imply that more than one PCR protocol should be used for diagnosis in holoendemic areas.

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • N. Oster
    • 1
    • 2
  • I. Z. Abdel-Aziz
    • 1
    • 3
  • A. Stich
    • 1
    • 4
  • B. Coulibaly
    • 5
  • B. Kouyatè
    • 5
  • K. T. Andrews
    • 1
    • 6
  • J. E. McLean
    • 1
  • M. Lanzer
    • 1
  1. 1.Hygiene Institut, Abteilung ParasitologieUniversitätsklinikum HeidelbergHeidelbergGermany
  2. 2.MurgGermany
  3. 3.Department of Parasitology, Faculty of MedicineCairo UniversityGizaEgypt
  4. 4.Missionsärztliches InstitutAG Tropenmedizin und SeuchenbekämpfungWurzburgGermany
  5. 5.Centre de Recherche en Santéà Nouna NounaBurkina Faso
  6. 6.Clinical Tropical Medicine LaboratoryThe Queensland Institute of Medical ResearchAustralia