Original Paper

Parasitology Research

, Volume 97, Issue 6, pp 527-534

First online:

Fasciola gigantica cathepsin-L cysteine proteinase in the detection of early experimental fasciolosis in ruminants

  • S. C. YadavAffiliated withDivision of Parasitology, IVRI
  • , M. SainiAffiliated withDivision of Parasitology, IVRIDivision of Biochemistry and Food Science, IVRI
  • , O. K. RainaAffiliated withDivision of Parasitology, IVRI Email author 
  • , P. A. NambiAffiliated withDivision of Parasitology, IVRI
  • , K. JadavAffiliated withDivision of Parasitology, IVRI
  • , D. SrivenyAffiliated withDivision of Parasitology, IVRI

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Cathepsin-L cysteine proteinase was purified from Fasciola gigantica regurgitant by two-step alcoholic fractionation, followed by ion-exchange chromatography. The purification strategy was evolved to eliminate other contaminating proteins co-precipitating with the purified proteinase during alcoholic fractionation. The enzyme was stable on long-term storage at –20°C rendering it more suitable for field diagnostic use. The purified cathepsin-L cysteine proteinase was assayed for detection of F. gigantica experimental infection in sheep and buffaloes and could detect infection, as early as 4 weeks post-infection by ELISA, Western blotting and Dipstick ELISA. The 28-kDa cathepsin-L cysteine proteinase seems a promising antigen for the diagnosis of tropical fasciolosis in domestic animals.