Parasitology Research

, Volume 97, Issue 2, pp 136–140

In vivo and in vitro analysis of chloroquine resistance in Plasmodium falciparum isolates from Senegal

  • Ousmane Sarr
  • Alissa Myrick
  • Johanna Daily
  • Bernard M. Diop
  • Therese Dieng
  • Omar Ndir
  • Pape Salif Sow
  • Souleymane Mboup
  • Dyann F. Wirth
Original Paper

DOI: 10.1007/s00436-005-1406-7

Cite this article as:
Sarr, O., Myrick, A., Daily, J. et al. Parasitol Res (2005) 97: 136. doi:10.1007/s00436-005-1406-7

Abstract

To determine the predictive value of chloroquine (CQ) resistance markers in Senegal, Plasmodium falciparum DNA polymorphisms in pfmdr1and pfcrt were examined in relation to clinical outcome. Despite CQ treatment, 17% of patients had parasitemia after 28 days. Examination of molecular markers of CQ resistance revealed that 64% of all isolates had the T76 resistant allele at the pfcrt locus, while 30% carried the Y86 resistant allele at the pfmdr1 locus. The pfcrt T76 allele was present not only in all in vivo resistant isolates, 89% of in vitro resistant isolates, but also in 35% of in vitro sensitive isolates. The pfmdr1 N86Y polymorphism did not correlate with in vitro or in vivo CQ resistance. Our data suggest that the pfcrt T76 allele alone is required but not a sufficient predictor for in vivo CQ resistance.

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Ousmane Sarr
    • 1
  • Alissa Myrick
    • 2
    • 5
  • Johanna Daily
    • 2
  • Bernard M. Diop
    • 3
  • Therese Dieng
    • 4
  • Omar Ndir
    • 4
  • Pape Salif Sow
    • 3
  • Souleymane Mboup
    • 1
  • Dyann F. Wirth
    • 2
  1. 1.Laboratory of Bacteriology and VirologyHopital Aristide Le DantecDakarUSA
  2. 2.Department of Immunology and Infectious DiseasesHarvard School of Public HealthBostonUSA
  3. 3.Infectious Diseases ClinicFann HospitalDakarUSA
  4. 4.Laboratory of Parasitology, Faculty of Medicine and PharmacyCheikh Anta Diop UniversityDakarUSA
  5. 5.Division of Infectious Diseases, San Francisco General HospitalUniversity of California at San FranciscoSan FranciscoUSA