Parasitology Research

, 99:7

Infectivity for mice of Trypanosoma cruzi I and II strains isolated from different hosts

  • Marta Bértoli
  • Miriam Hitomi Andó
  • Max Jean De Ornelas Toledo
  • Silvana Marques De Araújo
  • Mônica Lúcia Gomes
Original Paper

DOI: 10.1007/s00436-005-0122-7

Cite this article as:
Bértoli, M., Andó, M.H., De Ornelas Toledo, M.J. et al. Parasitol Res (2006) 99: 7. doi:10.1007/s00436-005-0122-7

Abstract

In this paper, the infectivity for mice of Trypanosoma cruzi I and II strains isolated from sylvatic animals, triatomines, and humans is determined using fresh blood examination, hemoculture, culture of macerated organs, and polymerase chain reaction (PCR). Six strains were considered to have low infectivity (9.1–18.2%), five medium (27.3–45.4%), and one high (100.0%). Infectivity of T. cruzi strains isolated from sylvatic animals was significantly higher than that of strains isolated from humans and triatomines (p=0.0141). No significant difference was observed between the infectivity of T. cruzi I and II strains. The parasite was detected by fresh blood examination in one strain, by hemoculture and culture of macerated organs in four strains, and by PCR in all strains. We conclude that the infectivity is related to the host from which the strains were isolated, but the infectivity is not related to the genetic group of the parasite. We also conclude that the majority of the strains studied have low and medium infectivity for mice, and that PCR is an important tool to detect T. cruzi in strains with this biological characteristic.

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Marta Bértoli
    • 1
  • Miriam Hitomi Andó
    • 1
  • Max Jean De Ornelas Toledo
    • 1
  • Silvana Marques De Araújo
    • 1
  • Mônica Lúcia Gomes
    • 1
    • 2
  1. 1.Departamento de Análises Clínicas, Parasitologia BásicaUniversidade Estadual De MaringáMaringáBrazil
  2. 2.Laboratório de Doença de ChagasDepartamento de Análises Clínicas-Universidade Estadual de MaringáMaringáBrazil