, Volume 98, Issue 6, pp 588-592
Date: 31 Jan 2006

The anti-amoebic activity of some medicinal plants used by AIDS patients in southern Thailand

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The anti-amoebic activities of chloroform, methanol and water extracts from 12 Thai medicinal plants (39 extracts) commonly used by AIDS patients in southern Thailand were screened, at a concentration of 1,000 μg/ml, against Entamoeba histolytica strain HTH-56:MUTM and strain HM1:IMSS growing in vitro. The extracts were incubated with 2×105 E. histolytica trophozoites/ml of medium at 37°C under anaerobic conditions for 24 h. The cultures were examined with an inverted microscope and scored (1–4) according to the appearance and numbers of the trophozoites. The extracts that caused inhibition were selected and retested using the same conditions but with concentrations that ranged from 31.25 to 1,000 μg/ml using E. histolytica strain HM1:IMSS, and the IC50 values for each extract were calculated. The chloroform extracts from Alpinia galanga (IC50 55.2 μg/ml), Barleria lupulina (IC50 78.5 μg/ml), Boesenbergia pandurata (IC50 45.8 μg/ml), Piper betle (IC50 91.1 μg/ml) and Piper chaba (IC50 71.4 μg/ml) and the methanol extract from B. pandurata (IC50 57.6 μg/ml) were all classified as “active”, i.e. with an IC50 of less than 100 μg/ml, whereas those from Murraya paniculata (IC50 116.5 μg/ml) and Zingiber zerumbet (IC50 196.9 μg/ml) were classified as being “moderately active”. The IC50 of a standard drug, metronidazole, was 1.1 μg/ml.