Parasitology Research

, Volume 98, Issue 1, pp 67–74

Intramuscular immunization with p36(LACK) DNA vaccine induces IFN-γ production but does not protect BALB/c mice against Leishmania chagasi intravenous challenge

  • Eduardo A. Marques-da-Silva
  • Eduardo A. F. Coelho
  • Daniel C. O. Gomes
  • Márcia C. Vilela
  • Cássio Z. Masioli
  • Carlos A. P. Tavares
  • Ana Paula Fernandes
  • Luis Carlos C. Afonso
  • Simone A. Rezende
Original Paper

DOI: 10.1007/s00436-005-0008-8

Cite this article as:
Marques-da-Silva, E.A., Coelho, E.A.F., Gomes, D.C.O. et al. Parasitol Res (2005) 98: 67. doi:10.1007/s00436-005-0008-8

Abstract

Acute visceral leishmaniasis is a progressive disease caused by Leishmania chagasi in South America. The acquisition of immunity following infection suggests that vaccination is a feasible approach to protect against this disease. Since Leishmania homologue of receptors for activated C kinase (LACK) antigen is of particular interest as a vaccine candidate because of the prominent role it plays in the pathogenesis of experimental Leishmania major infection, we evaluated the potential of a p36(LACK) DNA vaccine in protecting BALB/c mice challenged with L. chagasi. In this study, mice received intramuscular (i.m.) or subcutaneous (s.c.) doses of LACK DNA vaccine. We evaluated the production of vaccine-induced cytokines and whether this immunization was able to reduce parasite load in liver and spleen. We detected a significant production of interferon gamma by splenocytes from i.m. vaccinated mice in response to L. chagasi antigen and to rLACK protein. However, we did not observe a reduction in parasite load neither in liver nor in the spleen of vaccinated animals. The lack of protection observed may be explained by a significant production of IL-10 induced by the vaccine.

Keywords

Leishmania chagasi Lack Vaccine IFN-γ IL-4 IL-10 

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Eduardo A. Marques-da-Silva
    • 1
  • Eduardo A. F. Coelho
    • 3
  • Daniel C. O. Gomes
    • 1
  • Márcia C. Vilela
    • 1
  • Cássio Z. Masioli
    • 1
  • Carlos A. P. Tavares
    • 2
  • Ana Paula Fernandes
    • 2
  • Luis Carlos C. Afonso
    • 1
  • Simone A. Rezende
    • 1
    • 5
  1. 1.Laboratório de Imunoparasitologia, DECBI/NUPEB-ICEB, Campus Morro do CruzeiroUniversidade Federal de Ouro PretoOuro PretoBrazil
  2. 2.Departamento de Bioquímica e Imunologia ICB,Belo HorizonteBrazil
  3. 3.Departamento de Microbiologia, Escola de FarmáciaUFMGBelo HorizonteBrazil
  4. 4.Setor de Patologia ClínicaCOLTEC–UFMGBelo HorizonteBrazil
  5. 5.Departamento de Análises Clínicas, Escola de FarmáciaUniversidade Federal de Ouro PretoOuro PretoBrazil