Parasitology Research

, Volume 91, Issue 2, pp 137–143

Study of specific IgG subclass antibodies for diagnosis of Gnathostoma spinigerum

  • Surang Nuchprayoon
  • Vivornpun Sanprasert
  • Montamas Suntravat
  • Kanyarat Kraivichian
  • Wilai Saksirisampant
  • Issarang Nuchprayoon
Original Paper

DOI: 10.1007/s00436-003-0947-x

Cite this article as:
Nuchprayoon, S., Sanprasert, V., Suntravat, M. et al. Parasitol Res (2003) 91: 137. doi:10.1007/s00436-003-0947-x

Abstract

Gnathostoma spinigerum infection is endemic in Thailand and many Asian countries. Current diagnosis is the skin test and enzyme-linked immunosorbent assay (ELISA) for IgG antibody against the G. spinigerum third-stage larvae (L3), but cross-reactivity is common. We evaluated the sensitivity and specificity of anti-G. spinigerum L3 IgG subclass antibodies for diagnosis of 43 patients with gnathostomiasis. The majority of patients with gnathostomiasis (91%) had eosinophilia. While the anti-G. spinigerum L3 IgG1 antibody provided the highest sensitivity (98%), the anti-G. spinigerum L3 IgG2 antibody had the highest specificity (88%). The ELISA that detected anti-G. spinigerum L3 IgG1 antibody could be a reliable laboratory screening test, while anti-G. spinigerum L3 IgG2 antibody could be used to confirm the diagnosis.

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Surang Nuchprayoon
    • 1
    • 3
  • Vivornpun Sanprasert
    • 4
  • Montamas Suntravat
    • 4
  • Kanyarat Kraivichian
    • 1
  • Wilai Saksirisampant
    • 1
  • Issarang Nuchprayoon
    • 2
    • 3
  1. 1.Department of Parasitology, Faculty of MedicineChulalongkorn UniversityBangkokThailand
  2. 2.Department of Pediatrics, Faculty of MedicineChulalongkorn UniversityBangkok Thailand
  3. 3.Chula Medical Research Center, Faculty of MedicineChulalongkorn UniversityBangkok Thailand
  4. 4.Medical Microbiology Program, Graduate SchoolChulalongkorn UniversityBangkok Thailand