Parasitology Research

, Volume 91, Issue 1, pp 60–67

Cloning and characterization of Taenia saginata paramyosin cDNA

  • Elizabeth Ferrer
  • Eva Moyano
  • Laura Benitez
  • Luis Miguel González
  • Denise Bryce
  • Mildred Foster-Cuevas
  • Iris Dávila
  • Maria Milagros Cortéz
  • Leslie J. S. Harrison
  • R. Michael E. Parkhouse
  • Teresa Gárate
Original Paper

DOI: 10.1007/s00436-003-0895-5

Cite this article as:
Ferrer, E., Moyano, E., Benitez, L. et al. Parasitol Res (2003) 91: 60. doi:10.1007/s00436-003-0895-5

Abstract

A λZAP-express cDNA library of Taenia saginata metacestodes was constructed. Antibody screening yielded a clone with an insert of 3,408 bp, an open reading frame of 2,589 bp, a deduced sequence of 863 amino acid and a molecular mass of 98.89 kDa. Alignments of the predicted amino acid sequence showed identity with paramyosins from several species: 98.8% with Taenia solium, 96.3% with Echinococcus.granulosus and about 70% with Schistosoma spp. The insert was expressed and purified. A collagen binding assay was performed which showed that T. saginata GST-paramyosin retained this property in a dose-dependent manner. Problems were encountered due to high backgrounds in serological assays in the homologous T. saginata system. However, the recombinant paramyosin was recognized by antibodies present in 31.6% of sera from T. solium seropositive cysticercosis patients and 100% of the sera from acute cysticercosis patients. The immunodominant epitope was the carboxyl-terminal fragment of the molecule.

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Elizabeth Ferrer
    • 1
  • Eva Moyano
    • 1
  • Laura Benitez
    • 1
    • 7
  • Luis Miguel González
    • 1
  • Denise Bryce
    • 2
  • Mildred Foster-Cuevas
    • 3
    • 8
  • Iris Dávila
    • 4
  • Maria Milagros Cortéz
    • 5
  • Leslie J. S. Harrison
    • 2
  • R. Michael E. Parkhouse
    • 6
  • Teresa Gárate
    • 1
  1. 1.Instituto de Salud Carlos IIICentro Nacional de MicrobiologíaMadridSpain
  2. 2.Department of Tropical Animal Health, Sir Alexander Robertson Centre for Tropical Veterinary Medicine, Easter Bush Veterinary CentreUniversity of EdinburghRoslinScotland
  3. 3.Institute for Animal HealthPirbright LaboratoriesWokingEngland
  4. 4.Departamento de ParasitologíaUniversidad de CaraboboValenciaVenezuela
  5. 5.Centro de Investigaciones Biomédicas (BIOMED) Universidad de CaraboboMaracayVenezuela
  6. 6.Instituto Gulbenkian de Ciéncia OeirasPortugal
  7. 7.Departamento de Microbiología-III, Facultad de BiologíaUniversidad Complutense (UCM)MadridSpain
  8. 8.Sir William Dunn School of PathologyUniversity of OxfordOxfordEngland