Parasitology Research

, Volume 90, Issue 5, pp 365–368

Molecular cloning and immunolocalization of the 17 kDa myoglobin of Clonorchis sinensis

  • Young-Bae Chung
  • Hyun-Jong Yang
  • Sung-Jong Hong
  • Shin-Yong Kang
  • Mejeong Lee
  • Tae Yun Kim
  • Min-Ho Choi
  • Jong-Yil Chai
  • Sung-Tae Hong
Original Paper

DOI: 10.1007/s00436-003-0837-2

Cite this article as:
Chung, YB., Yang, HJ., Hong, SJ. et al. Parasitol Res (2003) 90: 365. doi:10.1007/s00436-003-0837-2

Abstract

We purified the 17 kDa protein abundant in Clonorchis sinensis crude extracts. The N-terminal amino acid sequence of this protein was determined and an oligonucleotide probe synthesized. Using this probe, the cDNA encoding the protein was cloned and sequenced from the C. sinensis cDNA library. It was found to consist of a total of 150 amino acids and to have 41% conserved homology with the myoglobin of the trematodes Paramphistomum epiclitum and Isoparorchis hypselobagri. The gene product over-expressed in the bacterial system was purified and identified as the same molecule in the adult worms. BALB/c mouse sera raised against the adult 17 kDa protein revealed that this myoglobin was distributed throughout the parenchymal tissues except for the eggs and reproductive organs and that the protein may be involved in the survival of C. sinensis in the oxygen-depleted environment of the host.

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Young-Bae Chung
    • 1
  • Hyun-Jong Yang
    • 2
  • Sung-Jong Hong
    • 3
  • Shin-Yong Kang
    • 3
  • Mejeong Lee
    • 4
  • Tae Yun Kim
    • 4
  • Min-Ho Choi
    • 4
  • Jong-Yil Chai
    • 4
  • Sung-Tae Hong
    • 4
  1. 1.Department of Parasitology, College of MedicineCheju National UniversityJeju 690-756Korea
  2. 2.Department of Parasitology, College of MedicineEwha Woman's UniversitySeoul 158-710Korea
  3. 3.Department of ParasitologyChung-Ang University College of MedicineSeoul 156-756Korea
  4. 4.Department of ParasitologySeoul National University College of MedicineSeoul 110-799Korea