Journal of Cancer Research and Clinical Oncology

, Volume 127, Issue 7, pp 449–454

Induction and expression of cyclin D3 in human pancreatic cancer

Authors

  • Matthias P. A. Ebert
    • Department of Gastroenterology, Hepatology and Infectious Diseases, Otto-von-Guericke University of Magdeburg, 39120 Magdeburg, Germany Tel.: +49-391-6713156; Fax: +49-391-67190054 e-mail: Matthias.Ebert@medizin.uni-magdeburg.de
  • Sara Hernberg
    • Department of Gastroenterology, Hepatology and Infectious Diseases, Otto-von-Guericke University of Magdeburg, 39120 Magdeburg, Germany Tel.: +49-391-6713156; Fax: +49-391-67190054 e-mail: Matthias.Ebert@medizin.uni-magdeburg.de
  • Guo Fei
    • Department of Gastroenterology, Hepatology and Infectious Diseases, Otto-von-Guericke University of Magdeburg, 39120 Magdeburg, Germany Tel.: +49-391-6713156; Fax: +49-391-67190054 e-mail: Matthias.Ebert@medizin.uni-magdeburg.de
  • Armin Sokolowski
    • Institute of Clinical Chemistry and Pathobiochemistry, Otto-von-Guericke University of Magdeburg, 39120 Magdeburg, Germany
  • Hans U. Schulz
    • Department of General Surgery, Otto-von-Guericke University of Magdeburg, 39120 Magdeburg, Germany
  • Hans Lippert
    • Department of General Surgery, Otto-von-Guericke University of Magdeburg, 39120 Magdeburg, Germany
  • Peter Malfertheiner
    • Department of Gastroenterology, Hepatology and Infectious Diseases, Otto-von-Guericke University of Magdeburg, 39120 Magdeburg, Germany Tel.: +49-391-6713156; Fax: +49-391-67190054 e-mail: Matthias.Ebert@medizin.uni-magdeburg.de
ORIGINAL PAPER

DOI: 10.1007/s004320100235

Cite this article as:
Ebert, M., Hernberg, S., Fei, G. et al. J Cancer Res Clin Oncol (2001) 127: 449. doi:10.1007/s004320100235

Abstract

Purpose: Cyclins play a key role in the control and regulation of the cell cycle. The role of cyclins in the pathogenesis of pancreatic cancer is largely unknown. Methods: Using Northern blot analysis, polymerase chain reaction (PCR) and immunohistochemistry, we examined the expression of cyclins D1, D2, and D3 in human pancreatic cancer and studied the induction of these cyclins by growth factors in pancreatic cancer cell lines. Results: We now report that cyclin D1 and D3 mRNAs are expressed in human pancreatic cancer cell lines, and that the expression of cyclin D3 is enhanced in pancreatic cancer cells by amphiregulin, a member of the epidermal growth factor family. Cyclins D1 and D3 are also expressed in normal and malignant pancreatic tissues. However, while the normal pancreas and pancreatic cancers express cyclin D2 as determined by reverse-transcriptase PCR, we could not detect cyclin D2 mRNA by either Northern blot analysis or reverse transcriptase PCR in the two pancreatic cancer cell lines. Immunohistochemical analysis revealed the expression of cyclin D3 in pancreatic cancer cells. Conclusions: These findings suggest that D-type cyclins are differentially expressed in pancreatic cancer and that the aberrant activation of the EGF receptor in human pancreatic cancer by amphiregulin may lead to the progression of the cell cycle via induction of cyclin D3 expression, thus contributing to the growth advantage of these transformed cells.

Key words Cell cycleCyclinGrowth factorCancerPancreas

Copyright information

© Springer-Verlag Berlin Heidelberg 2001