Journal of Cancer Research and Clinical Oncology

, Volume 126, Issue 5, pp 271–279

Bone morphogenetic protein 2 (BMP-2) induces sequential changes of Id gene expression in the breast cancer cell line MCF-7

  • Joachim H. Clement
  • Nannette Marr
  • Anke Meissner
  • Manuela Schwalbe
  • Walter Sebald
  • Kay-Oliver Kliche
  • Klaus Höffken
  • Stefan Wölfl
ORIGINAL PAPER

DOI: 10.1007/s004320050342

Cite this article as:
Clement, J., Marr, N., Meissner, A. et al. J Cancer Res Clin Oncol (2000) 126: 271. doi:10.1007/s004320050342

Abstract

 Bone morphogenetic proteins (BMPs) are involved in the development of various organs including the mammary gland. They are well-regulated and act in a time-, concentration- and cell-type-specific manner. We found that BMP-2 is expressed in primary breast tumor tissue samples and in breast cancer cell lines. Hybridization of labeled cDNA, obtained from the breast cancer cell line MCF-7, against the Atlas human cDNA expression array revealed differential gene expression depending on BMP-2 treatment. The most prominent changes were observed for the helix-loop-helix proteins Id-1, Id-2 and Id-3. Id-1 expression had increased severalfold after 4 h and was even higher after 24 h. Id-2 and Id-3 were more strongly induced after 4 h and showed no further significant change after 24 h. Analysis of cell-cycle distribution revealed a marked increase of the sub-G1 phase after 48 h in serum-deprived cells. In the presence of BMP-2 no change was observed over 48 h indicating that BMP-2 does not induce apoptosis. In addition, expression of caspase-3 was reduced in BMP-2-treated cells after 24 h. In summary, our results clearly indicate that BMP-2 is a susceptibility factor keeping the cells ready for the integration of various other signals for cell progression.

Key words Tumor biologyBone morphogenetic protein 2Id proteinsExpression arrayBreast cancer

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Joachim H. Clement
    • 1
  • Nannette Marr
    • 2
  • Anke Meissner
    • 1
  • Manuela Schwalbe
    • 1
  • Walter Sebald
    • 3
  • Kay-Oliver Kliche
    • 1
  • Klaus Höffken
    • 1
  • Stefan Wölfl
    • 2
  1. 1.Department of Internal Medicine II, Friedrich Schiller University Jena, Erlanger Allee 101, D-07740 Jena e-mail: clement@polkim.med.uni-jena.de Tel.: +49-3641-939-214 Fax: +49-3641-939-542DE
  2. 2.Department of Cell and Molecular Biology, Hans-Knöll-Institut für Naturstoff-Forschung, Beutenbergstrasse 11, D-07745 JenaDE
  3. 3.Department of Physiological Chemistry II, Theodor-Boveri-Institute for Biosciences, University of Würzburg, Am Hubland, D-97074 Würzburg, GermanyDE