Journal of Cancer Research and Clinical Oncology

, Volume 139, Issue 10, pp 1735–1744

Expression of E-cadherin, Twist, and p53 and their prognostic value in patients with oral squamous cell carcinoma

  • Chi-Chen Fan
  • Tao-Yeuan Wang
  • Yen-An Cheng
  • Shih Sheng Jiang
  • Chien-Wen Cheng
  • Alan Yueh-Luen Lee
  • Ting-Yu Kao
Original Paper

DOI: 10.1007/s00432-013-1499-9

Cite this article as:
Fan, CC., Wang, TY., Cheng, YA. et al. J Cancer Res Clin Oncol (2013) 139: 1735. doi:10.1007/s00432-013-1499-9

Abstract

Purpose

Epithelial–mesenchymal transition (EMT) and p53 play important roles in controlling cancer invasion and metastasis. However, discrepancies still exist in the relationship between the expression of an epithelial marker E-cadherin and predicting short survival of patients in many types of cancer. In this study, we aimed to determine the levels of E-cadherin, Twist, and p53 in tumor tissues from patients with oral squamous cell carcinoma (OSCC) and their clinical significances.

Methods

The protein expression of 112 OSCC tumor and 16 benign tissues was examined by immunohistochemistry staining. Overall survival rates of 112 OSCC patients were measured using Kaplan–Meier estimates and the log-rank tests.

Results

E-cadherin and p53 downregulation were found in 70 of 112 (62.5 %) and 66 of 112 (59.0 %), respectively, and Twist overexpression was found in 72 of 112 (64.3 %) studied cases of OSCC patients. Expression of E-cadherin was significantly associated with tumor location (P = 0.004) and mortality (P = 0.010). Patients with lower E-cadherin expression (P = 0.024), betel quid chewing (P = 0.006), smoking (P = 0.001), tumor size >2 cm (P = 0.001), advanced tumor stage (P = 0.043), and recurrence (P < 0.001) exhibited a poorer outcome. Multivariate analysis showed that E-cadherin is an independent marker for survival prediction. Additionally, low E-cadherin expression is significantly correlated with low p53 expression.

Conclusions

E-cadherin is an independent marker for survival prediction in OSCC. Co-evaluation of E-cadherin and p53 expression might be a valuable tool for predicting OSCC patient outcome.

Keywords

E-cadherinEMTp53Prognostic markerOral cancer

Abbreviations

EMT

Epithelial–mesenchymal transition

IHC

Immunohistochemistry

OSCC

Oral squamous cell carcinoma

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Chi-Chen Fan
    • 1
    • 2
  • Tao-Yeuan Wang
    • 3
  • Yen-An Cheng
    • 2
  • Shih Sheng Jiang
    • 4
  • Chien-Wen Cheng
    • 4
  • Alan Yueh-Luen Lee
    • 4
  • Ting-Yu Kao
    • 2
  1. 1.Laboratory of PhysiologyMackay Memorial HospitalTaipeiTaiwan
  2. 2.Department of Medical Laboratory Science and BiotechnologyYuanpei UniversityHsinchuTaiwan
  3. 3.Department of Pathology, Mackay Memorial HospitalMackay Medical College, Mackay Medicine, Nursing and Management CollegeTaipeiTaiwan
  4. 4.National Institute of Cancer ResearchNational Health Research InstitutesZhunan, MiaoliTaiwan