Journal of Cancer Research and Clinical Oncology

, Volume 138, Issue 7, pp 1221–1229

Effect of shRNA targeting survivin on ovarian cancer

  • Jun Xing
  • Chang-Ru Jia
  • Yan Wang
  • Jing Guo
  • Yan Cai
Original Paper

DOI: 10.1007/s00432-012-1196-0

Cite this article as:
Xing, J., Jia, CR., Wang, Y. et al. J Cancer Res Clin Oncol (2012) 138: 1221. doi:10.1007/s00432-012-1196-0

Abstract

Purpose

This study investigated the effect of shRNA targeting survivin on cultured ovarian cancer cells and on a murine ovarian cancer xenograft.

Methods

An RNAi plasmid for survivin was transfected into SKOV3 cells, and the effect of shRNA targeting survivin on the expression of survivin was determined. Transmission electron microscopy (TEM), flow cytometry, and TUNEL staining were used to assess apoptosis. The MTT assay was used to measure cell growth and changes in cisplatin sensitivity. SKOV3 cells were injected into nude mice, and the effect of shRNA targeting the survivin gene on tumor growth was assessed.

Results

SKOV3 cells transfected with an RNAi plasmid against survivin had increased apoptosis and slower growth. At the molecular level, these cells also had lower expression of survivin. Nude mice inoculated with SKOV3 cells developed cancers, and treatment with shRNA targeting survivin markedly inhibited the growth of these cancers with no obvious side effects.

Conclusions

Our studies of SKOV3 cells and ovarian cancer xenografts in nude mice indicate that shRNA targeting survivin has potential for the treatment of ovarian cancer.

Keywords

RNA interference Survivin SKOV3 cells Ovarian cancer Matrix metalloproteinase 2 Tissue inhibitor of metalloproteinase 1 Apoptosis Cisplatin 

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Jun Xing
    • 1
  • Chang-Ru Jia
    • 2
  • Yan Wang
    • 3
  • Jing Guo
    • 4
  • Yan Cai
    • 2
  1. 1.Department of Gastrointestinal SurgeryThe Third Hospital of Harbin Medical UniversityNan Gang DistrictPeople’s Republic of China
  2. 2.Department of ObstetricsThe Fourth Hospital of Harbin Medical UniversityNan Gang DistrictPeople’s Republic of China
  3. 3.Department of Internal MedicineThe Third Hospital of Harbin Medical UniversityNan Gang DistrictPeople’s Republic of China
  4. 4.Department of Obstetrics and GynecologyJian Hua HospitalJian Hua DistrictPeople’s Republic of China