Journal of Cancer Research and Clinical Oncology

, Volume 138, Issue 1, pp 153–161

Up-regulation of microRNA-155 promotes cancer cell invasion and predicts poor survival of hepatocellular carcinoma following liver transplantation

  • Zhong-Bo Han
  • Hong-Yuan Chen
  • Jun-Wei Fan
  • Jun-Yi Wu
  • Hua-Mei Tang
  • Zhi-Hai Peng
Original Paper

DOI: 10.1007/s00432-011-1076-z

Cite this article as:
Han, ZB., Chen, HY., Fan, JW. et al. J Cancer Res Clin Oncol (2012) 138: 153. doi:10.1007/s00432-011-1076-z

Abstract

Purpose

MicroRNAs play important roles in cancer development, progression, and metastasis. The aim of this study was to determine whether altered microRNA-155 expression is associated with hepatocellular carcinoma (HCC) recurrence and prognosis following orthotopic liver transplantation (OLT).

Methods

Tissue specimens from 100 HCC patients following OLT were recruited. MicroRNA-155 expression levels were detected using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Kaplan–Meier and Cox proportional regression analyses were utilized to determine the association of microRNA-155 expression with survival of patients. MicroRNA-155 expression levels of two HCC cell lines (HepG2 and SMMC-7721) and normal liver tissue were quantified using qRT-PCR. The potential function of miR-155 on invasiveness was evaluated in the above HCC cell lines.

Results

We found that microRNA-155 expression levels were high in tumor tissues in patients with post-OLT HCC recurrence (n = 45) compared with those in patients with non-recurrence (n = 55) (P = 0.001) and correlated with micro-vascular invasion of HCC tissue samples (P = 0.001). Patients with higher miR-155 expression had significantly poorer recurrence-free survival (RFS, log rank P < 0.001) and overall survival (OS, log rank P < 0.001). Multivariate analysis revealed that high miR-155 expression was an independent predictor of poor prognosis (HR 2.748, P = 0.001 for RFS; HR 5.752, P < 0.001 for OS). In addition, the invasiveness of HCC cells was significantly increased by higher microRNA-155 expression.

Conclusions

MicroRNA-155 is a candidate oncogenic microRNA and plays an important role in promoting HCC cells invasion. Our findings suggest that microRNA-155 may serve as a novel biomarker for tumor recurrence and survival of HCC patients following OLT.

Keywords

MicroRNA-155Hepatocellular carcinomaRecurrencePrognosisLiver transplantation

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Zhong-Bo Han
    • 1
  • Hong-Yuan Chen
    • 1
  • Jun-Wei Fan
    • 1
  • Jun-Yi Wu
    • 1
  • Hua-Mei Tang
    • 2
  • Zhi-Hai Peng
    • 1
  1. 1.Department of General Surgery, Shanghai First People’s Hospital, School of MedicineShanghai Jiao Tong UniversityShanghaiChina
  2. 2.Department of Pathology, Shanghai First People’s Hospital, School of MedicineShanghai Jiao Tong UniversityShanghaiChina