Journal of Cancer Research and Clinical Oncology

, Volume 138, Issue 1, pp 49–56

The microRNA-processing enzymes: Drosha and Dicer can predict prognosis of nasopharyngeal carcinoma

Authors

  • Xiaofang Guo
    • Cancer Research InstituteCentral South University
    • Changsha Medical University
  • Qianjin Liao
    • Cancer Research InstituteCentral South University
    • Cancer Research InstituteUniversity of South China
  • Pan Chen
    • Cancer Research InstituteCentral South University
  • Xiayu Li
    • The Third Xiangya HospitalCentral South University
  • Wei Xiong
    • Cancer Research InstituteCentral South University
  • Jian Ma
    • Cancer Research InstituteCentral South University
  • Xiaoling Li
    • Cancer Research InstituteCentral South University
  • Zhaohui Luo
    • Cancer Research InstituteCentral South University
  • Hailin Tang
    • Cancer Research InstituteCentral South University
    • Cancer Research InstituteUniversity of South China
  • Min Deng
    • Cancer Research InstituteCentral South University
    • Cancer Research InstituteUniversity of South China
  • Yin Zheng
    • Cancer Research InstituteCentral South University
  • Rong Wang
    • Cancer Research InstituteCentral South University
    • Cancer Research InstituteCentral South University
    • Cancer Research InstituteCentral South University
Original Paper

DOI: 10.1007/s00432-011-1058-1

Cite this article as:
Guo, X., Liao, Q., Chen, P. et al. J Cancer Res Clin Oncol (2012) 138: 49. doi:10.1007/s00432-011-1058-1

Abstract

Purpose

Dysregulation of microRNA (miRNA) metabolism has been observed in a variety of human cancers, but the expression patterns of the enzymes responsible for generating miRNAs remain largely unexplored. In this study, we investigated the expression profiles of the two most important enzymes of the miRNA machinery, Drosha and Dicer, which were closely correlated with nasopharyngeal carcinoma (NPC) and patient survival.

Methods

Dicer and Drosha mRNA levels were detected by quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) using 24 NPC tissues, 7 normal nasopharyngeal epithelium samples (NPE) and NPC cell lines. In addition, protein levels were detected by immunohistochemistry (IHC) using an NPC tissue microarray (TMA), which include 251 NPC and 105 NPE cases. For some NPC patients can not be contacted, the survival data were available only for 146 patients. Kaplan–Meier analysis was performed, and the chi-square and log-rank tests were used to detect significance levels using SPSS 15.0 software.

Results

The mean level of Dicer and Drosha mRNA were significantly down-regulated in NPC tissue specimens and cell lines when compared with controls. The low levels of Dicer and Drosha protein were frequently seen in NPC, and the low expression of Dicer and Drosha protein was significantly correlated with shorter progression-free survival (PFS) and overall survival (OS) of NPC patients.

Conclusions

We observed that Drosha and Dicer expression was dysregulation in NPC compared with healthy control samples and was significantly correlated with shorter PFS and OS of NPC patients. Therefore, we hypothesise that the expression levels of Dicer and Drosha could be used as potential prognostic biomarkers for NPC.

Keywords

Dicer Drosha Nasopharyngeal carcinoma Progression-free survival Overall survival

Copyright information

© Springer-Verlag 2011