Journal of Cancer Research and Clinical Oncology

, Volume 137, Issue 8, pp 1193–1201

Prognostic value of intercellular adhesion molecule (ICAM)-1 expression in breast cancer

  • Christine Schröder
  • Isabell Witzel
  • Volkmar Müller
  • Sylke Krenkel
  • Ralph M. Wirtz
  • Fritz Jänicke
  • Udo Schumacher
  • Karin Milde-Langosch
Original Paper

DOI: 10.1007/s00432-011-0984-2

Cite this article as:
Schröder, C., Witzel, I., Müller, V. et al. J Cancer Res Clin Oncol (2011) 137: 1193. doi:10.1007/s00432-011-0984-2

Abstract

Purpose

The intercellular adhesion molecule (ICAM)-1 is expressed on many cell types including endothelial cells and different cancer cell entities. Experimental data strongly indicate that ICAM-1 can activate intracellular signalling pathways in cancer cells leading to enhanced cell motility, invasion and metastasis. Yet, little is known about the role of ICAM-1 expression during malignant progression in breast cancer patients.

Methods

We investigated ICAM-1 protein and mRNA expression in two partly overlapping cohorts of breast cancer patients. ICAM-1 protein was detected by Western blot analysis in 104 cases and verified by immunohistochemistry. Additionally, ICAM-1 mRNA microarray data from 169 tumours were analysed.

Results

With both methods, high ICAM-1 expression was significantly associated with a poorly differentiated phenotype, a negative estrogen receptor (ER) status and positive lymph node involvement. In addition, there was a significant prognostic impact of ICAM-1 protein overexpression on recurrence-free survival (HR = 2.82, P = 0.023), which was most pronounced in ER-negative tumours. ICAM-1 mRNA overexpression was associated with high urokinase plasminogen activator (uPA) and uPA-inhibitor protein 1 (PAI 1) protein and mRNA levels as well as high Ki67 protein and vascular endothelial growth factor (VEGF) mRNA expression.

Conclusions

In our group of patients, ICAM-1 expression was associated with a more aggressive tumour phenotype. Because of its association with malignant progression, ICAM-1 might represent a new target in the treatment of breast cancer patients.

Keywords

ICAM-1 Breast cancer Prognosis Microarray analysis Western blots Immunohistochemistry 

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Christine Schröder
    • 1
  • Isabell Witzel
    • 2
  • Volkmar Müller
    • 2
  • Sylke Krenkel
    • 2
  • Ralph M. Wirtz
    • 3
  • Fritz Jänicke
    • 2
  • Udo Schumacher
    • 1
  • Karin Milde-Langosch
    • 2
  1. 1.Department of AnatomyUniversity Medical Centre Hamburg-Eppendorf (UKE)HamburgGermany
  2. 2.Department of GynaecologyUniversity Medical Centre Hamburg-Eppendorf (UKE)HamburgGermany
  3. 3.CEOSTRATIFYER Molecular Pathology GmbHKölnGermany