Journal of Cancer Research and Clinical Oncology

, Volume 137, Issue 4, pp 651–658

Preoperative serum retinol-binding protein 4 is associated with the prognosis of patients with hepatocellular carcinoma after curative resection

Authors

  • Dan-Dan Wang
    • Department of EndocrinologyPunan Hospital
  • Yi-Ming Zhao
    • Liver Cancer Institute, Zhongshan HospitalFudan University
    • Liver Cancer Institute, Zhongshan HospitalFudan University
  • Guang Ren
    • Department of RadiologyHuashan Hospital, Fudan University
  • Fei Wang
    • Department of RadiologyTenth People’s Hospital, Tongji University
  • Zu-Guang Xia
    • Department of HematologyNinth People’s Hospital, Shanghai Jiaotong University School of Medicine
  • Xi-Long Wang
    • Liver Cancer Institute, Zhongshan HospitalFudan University
  • Tao Zhang
    • Department of RadiologySecond Affiliated Hospital, Xuzhou Medical College
  • Qi Pan
    • Liver Cancer Institute, Zhongshan HospitalFudan University
  • Zhi Dai
    • Liver Cancer Institute, Zhongshan HospitalFudan University
  • Ju-Ping Chen
    • Department of EndocrinologyPunan Hospital
  • Hai-Yan Dai
    • Department of Gynecology and ObstetricsPunan Hospital
  • Wei Zhang
    • Gynecology and Obstetrics Institute, Gynecology and Obstetrics HospitalFudan University
  • Hong-Wei He
    • Liver Cancer Institute, Zhongshan HospitalFudan University
  • Jia-Min Zhou
    • Liver Cancer Institute, Zhongshan HospitalFudan University
    • Department of RadiologyTenth People’s Hospital, Tongji University
  • Jian Zhou
    • Liver Cancer Institute, Zhongshan HospitalFudan University
  • Jia Fan
    • Liver Cancer Institute, Zhongshan HospitalFudan University
  • Zhao-You Tang
    • Liver Cancer Institute, Zhongshan HospitalFudan University
Original Paper

DOI: 10.1007/s00432-010-0927-3

Cite this article as:
Wang, D., Zhao, Y., Wang, L. et al. J Cancer Res Clin Oncol (2011) 137: 651. doi:10.1007/s00432-010-0927-3

Abstract

Purpose

Metabolic syndrome and insulin resistance have been linked to increased risk of occurrence and mortality of hepatocellular carcinoma (HCC). Recently, retinol-binding protein 4 (RBP4) was clarified as a specific serological marker of insulin resistance. The aim of this study was to determine whether serum RBP4 could be used as a potential marker for predicting prognosis in patients with HCC after curative resection.

Methods

Western immunoblotting and Enzyme-linked immunosorbent assay were used to measure the RBP4 expression in cell lines, supernatant, and serum. Serum RBP4 levels were compared with clinicopathological features and outcomes of patients with HCC. Furthermore, we investigated the impact of serum RBP4 level, serum C-peptide level, and HOMA-IR on overall survival (OS) and disease-free survival (DFS) of patients with HCC in the training cohort (156 patients with HCC), and then were validated in the validation cohort (105 patients with HCC).

Results

RBP4 protein overexpressed in HCC cell lines compared with normal liver cell line (P < 0.001) and correlated with metastatic potential. Serum RBP4 levels were associated with OS [hazard ratio (HR) = 2.208, P < 0.001] and DFS (HR = 1.878, P = 0.029) of patients with HCC. By multivariate analysis, the serum RBP4 level was identified as an independent factor for OS (HR = 2.170, P = 0.004) and DFS (HR = 1.769, P = 0.037) of patients with HCC. The prognostic value of serum RBP4 level was confirmed in the validation cohort.

Conclusions

The serum RBP4 level is potential to be a useful prognostic factor for HCC after curative resection.

Keywords

Retinol-binding proteinHepatocellular carcinomaSerumPrognosis

Supplementary material

432_2010_927_MOESM1_ESM.docx (15 kb)
Supplementary material 1 (DOCX 15 kb)
432_2010_927_MOESM2_ESM.tif (1 mb)
Supplemental Fig. S1 In training cohort, Stratified survival curve were drawn according to absence or presence of liver cirrhosis (A-D) and TNM stage (E-H) to further investigate the prognostic value of serum RBP4 in HCC patients (TIFF 1048 kb)
432_2010_927_MOESM3_ESM.tif (262 kb)
Supplemental Fig. S2 With the cutoff used in training cohort, prognostic significance of serum RBP4 level for OS (A) and DFS (B) were confirmed in the validation cohort (TIFF 262 kb)

Copyright information

© Springer-Verlag 2010