Journal of Cancer Research and Clinical Oncology

, Volume 137, Issue 4, pp 651–658

Preoperative serum retinol-binding protein 4 is associated with the prognosis of patients with hepatocellular carcinoma after curative resection

  • Dan-Dan Wang
  • Yi-Ming Zhao
  • Lu Wang
  • Guang Ren
  • Fei Wang
  • Zu-Guang Xia
  • Xi-Long Wang
  • Tao Zhang
  • Qi Pan
  • Zhi Dai
  • Ju-Ping Chen
  • Hai-Yan Dai
  • Wei Zhang
  • Hong-Wei He
  • Jia-Min Zhou
  • Guang-Yu Tang
  • Jian Zhou
  • Jia Fan
  • Zhao-You Tang
Original Paper

DOI: 10.1007/s00432-010-0927-3

Cite this article as:
Wang, D., Zhao, Y., Wang, L. et al. J Cancer Res Clin Oncol (2011) 137: 651. doi:10.1007/s00432-010-0927-3

Abstract

Purpose

Metabolic syndrome and insulin resistance have been linked to increased risk of occurrence and mortality of hepatocellular carcinoma (HCC). Recently, retinol-binding protein 4 (RBP4) was clarified as a specific serological marker of insulin resistance. The aim of this study was to determine whether serum RBP4 could be used as a potential marker for predicting prognosis in patients with HCC after curative resection.

Methods

Western immunoblotting and Enzyme-linked immunosorbent assay were used to measure the RBP4 expression in cell lines, supernatant, and serum. Serum RBP4 levels were compared with clinicopathological features and outcomes of patients with HCC. Furthermore, we investigated the impact of serum RBP4 level, serum C-peptide level, and HOMA-IR on overall survival (OS) and disease-free survival (DFS) of patients with HCC in the training cohort (156 patients with HCC), and then were validated in the validation cohort (105 patients with HCC).

Results

RBP4 protein overexpressed in HCC cell lines compared with normal liver cell line (P < 0.001) and correlated with metastatic potential. Serum RBP4 levels were associated with OS [hazard ratio (HR) = 2.208, P < 0.001] and DFS (HR = 1.878, P = 0.029) of patients with HCC. By multivariate analysis, the serum RBP4 level was identified as an independent factor for OS (HR = 2.170, P = 0.004) and DFS (HR = 1.769, P = 0.037) of patients with HCC. The prognostic value of serum RBP4 level was confirmed in the validation cohort.

Conclusions

The serum RBP4 level is potential to be a useful prognostic factor for HCC after curative resection.

Keywords

Retinol-binding proteinHepatocellular carcinomaSerumPrognosis

Supplementary material

432_2010_927_MOESM1_ESM.docx (15 kb)
Supplementary material 1 (DOCX 15 kb)
432_2010_927_MOESM2_ESM.tif (1 mb)
Supplemental Fig. S1 In training cohort, Stratified survival curve were drawn according to absence or presence of liver cirrhosis (A-D) and TNM stage (E-H) to further investigate the prognostic value of serum RBP4 in HCC patients (TIFF 1048 kb)
432_2010_927_MOESM3_ESM.tif (262 kb)
Supplemental Fig. S2 With the cutoff used in training cohort, prognostic significance of serum RBP4 level for OS (A) and DFS (B) were confirmed in the validation cohort (TIFF 262 kb)

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Dan-Dan Wang
    • 1
  • Yi-Ming Zhao
    • 8
  • Lu Wang
    • 8
  • Guang Ren
    • 3
  • Fei Wang
    • 6
  • Zu-Guang Xia
    • 4
  • Xi-Long Wang
    • 8
  • Tao Zhang
    • 5
  • Qi Pan
    • 8
  • Zhi Dai
    • 8
  • Ju-Ping Chen
    • 1
  • Hai-Yan Dai
    • 2
  • Wei Zhang
    • 7
  • Hong-Wei He
    • 8
  • Jia-Min Zhou
    • 8
  • Guang-Yu Tang
    • 6
  • Jian Zhou
    • 8
  • Jia Fan
    • 8
  • Zhao-You Tang
    • 8
  1. 1.Department of EndocrinologyPunan HospitalShanghaiChina
  2. 2.Department of Gynecology and ObstetricsPunan HospitalShanghaiChina
  3. 3.Department of RadiologyHuashan Hospital, Fudan UniversityShanghaiChina
  4. 4.Department of HematologyNinth People’s Hospital, Shanghai Jiaotong University School of MedicineShanghaiChina
  5. 5.Department of RadiologySecond Affiliated Hospital, Xuzhou Medical CollegeXuzhouChina
  6. 6.Department of RadiologyTenth People’s Hospital, Tongji UniversityShanghaiChina
  7. 7.Gynecology and Obstetrics Institute, Gynecology and Obstetrics HospitalFudan UniversityShanghaiChina
  8. 8.Liver Cancer Institute, Zhongshan HospitalFudan UniversityShanghaiChina