Journal of Cancer Research and Clinical Oncology

, Volume 137, Issue 3, pp 441–453

L-methionine-induced alterations in molecular signatures in MCF-7 and LNCaP cancer cells

  • Maximo A. Benavides
  • Dong Hu
  • Marie Kristine Baraoidan
  • Annette Bruno
  • Pan Du
  • Simon Lin
  • Wancai Yang
  • Kirby I. Bland
  • William E. Grizzle
  • Maarten C. Bosland
Original Paper

DOI: 10.1007/s00432-010-0897-5

Cite this article as:
Benavides, M.A., Hu, D., Baraoidan, M.K. et al. J Cancer Res Clin Oncol (2011) 137: 441. doi:10.1007/s00432-010-0897-5

Abstract

Background

Methionine inhibits proliferation of breast and prostate cancer cells. Here, we determined the influence of L-methionine on functional molecular signatures in these cell lines.

Methods

MCF-7 and LNCaP cells were treated with L-methionine (5 mg/ml) for 72 h. Changes in molecular signatures of these cells were examined by microarray analysis of 15,814 probes in triplicate experiments.

Results

In LNCaP cells, 325 genes were up-regulated by methionine, and 517 genes down-regulated. In MCF-7 cells, 86 genes were up-regulated and 135 genes down-regulated. Ninety-eight genes were regulated in the same direction by methionine in both cells lines, and five other genes were changed in expression in opposite directions.

Conclusion

Several of the up-regulated genes encode proteins involved in cellular redox regulation, suggesting that methionine may enhance antioxidant mechanisms. Many of the down-regulated genes belong to protein kinase families that may be related to the anti-proliferative effects of methionine on breast and prostate cancer cells.

Keywords

Methionine Gene expression Prostate cancer Breast cancer MCF-7 cells LNCaP cells 

Supplementary material

432_2010_897_MOESM1_ESM.pdf (208 kb)
Supplemental Table 1: Genes with altered expression in LNCaP cells treated with L-methionine 5 mg/ml for 72 h. Significant changes in expression were observed in a total of 842 genes out of 15,814 probes examined, with 325 genes up-regulated and 517 genes down-regulated. (PDF 208 kb)
432_2010_897_MOESM2_ESM.pdf (80 kb)
Supplemental Table 2: Genes with altered expression in MCF-7 cells treated with L-methionine 5 mg/ml for 72 h. Significant changes in expression were observed in a total of 221 genes out of 15,814 probes examined, with 86 genes were up-regulated and 135 genes down-regulated. (PDF 80 kb)

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Maximo A. Benavides
    • 1
  • Dong Hu
    • 1
  • Marie Kristine Baraoidan
    • 2
  • Annette Bruno
    • 2
  • Pan Du
    • 3
  • Simon Lin
    • 3
  • Wancai Yang
    • 1
  • Kirby I. Bland
    • 4
  • William E. Grizzle
    • 5
  • Maarten C. Bosland
    • 1
  1. 1.Department of Pathology, College of MedicineUniversity of Illinois at ChicagoChicagoUSA
  2. 2.DNA Service Laboratory, Research Resources CenterUniversity of Illinois at ChicagoChicagoUSA
  3. 3.Biomedical Informatics Center, Robert H. Lurie Comprehensive Cancer CenterNorthwestern UniversityChicagoUSA
  4. 4.Department of SurgeryUniversity of Alabama at Birmingham School of MedicineBirminghamUSA
  5. 5.Department of PathologyUniversity of Alabama at Birmingham School of MedicineBirminghamUSA

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