Journal of Cancer Research and Clinical Oncology

, Volume 136, Issue 11, pp 1641–1649

Knockdown of Li-cadherin increases metastatic behaviors of LoVo cells

Original Paper

DOI: 10.1007/s00432-010-0822-y

Cite this article as:
Yu, QF., Dong, WG. & Ren, JL. J Cancer Res Clin Oncol (2010) 136: 1641. doi:10.1007/s00432-010-0822-y



The aim of this study was to investigate the role of Li-cadherin in invasion and metastasis in LoVo cells.


We applied RNA interference mediated downregulation of Li-cadherin expression in LoVo cells. Li-cadherin expression in LoVo cells was examined by semiquantitative polymerase chain reaction, immunofluorescence, western blot, and immunoprecipitation, respectively. Effect of suppression of Li-cadherin expression on cell migration, invasion, and adhesion was detected by wound healing assay, migration assay, invasion assay, and adhesion assay. Expression and activity of MMP-2 and MMP-9 were analyzed by gelatin zymography.


Cell migration, invasion, and adhesion were increased concomitantly with the reduction in Li-cadherin protein expression. Furthermore, downregulation of Li-cadherin expression induced secretion of proMMP-9, active MMP-9 and active MMP-2.


This study suggests that silencing Li-cadherin has positive actions in the processes of LoVo cells invasion and metastasis, and the interactions among MMP-2, MMP-9, and Li-cadherin participate in the multiple steps of invasion and metastasis in LoVo colorectal cancer cells.


Li-cadherinRNA interferenceColorectal cancerMigrationInvasionAdhesion

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.Department of GastroenterologySecond Affiliated Hospital of Nanchang UniversityNanchangChina
  2. 2.Department of GastroenterologyRenmin Hospital of Wuhan UniversityWuhanPeople’s Republic of China
  3. 3.Department of Gastroenterology, Zhongshan HospitalXiamen UniversityXiamenChina