Journal of Cancer Research and Clinical Oncology

, Volume 136, Issue 5, pp 677–684

Overexpression of high-mobility group box 1 correlates with tumor progression and poor prognosis in human colorectal carcinoma

Authors

  • Xingjun Yao
    • Department of SurgeryThe First Affiliated Hospital of Jilin University
  • Gang Zhao
    • Department of SurgeryThe First Affiliated Hospital of Jilin University
  • Hongfa Yang
    • Department of SurgeryThe First Affiliated Hospital of Jilin University
  • Xinyu Hong
    • Department of SurgeryThe First Affiliated Hospital of Jilin University
  • Li Bie
    • Department of SurgeryThe First Affiliated Hospital of Jilin University
    • Department of SurgeryThe First Affiliated Hospital of Jilin University
Original Paper

DOI: 10.1007/s00432-009-0706-1

Cite this article as:
Yao, X., Zhao, G., Yang, H. et al. J Cancer Res Clin Oncol (2010) 136: 677. doi:10.1007/s00432-009-0706-1

Abstract

Purpose

High-mobility group box 1 (HMGB1) has been implicated in a variety of biologically important processes, including transcription, DNA repair, V(D)J recombination, differentiation, development, and extracellular signaling. The increased expression of HMGB1 has been described in colorectal cancer (CRC). However, there is no report about the correlation of HMGB1 with clinicopathologic features, including the survival of patients with CRC.

Methods

In present study, we investigated HMGB1 expression and its prognostic significance in CRC by performing immunohistochemical analysis, using a total of 192 paraffin-embedded archival CRC samples. Moreover, disruption of endogenous HMGB1 protein through a siRNA knockdown technique was performed to investigate the possible role of HMGB1 in the process of tumor invasion and metastasis.

Results

Overexpression of HMGB1 was shown in 55.7% cases. Statistical analysis showed that HMGB1 expression was positively correlated with tumor invasion (P = 0.048), lymph node metastasis (P = 0.011), distant metastasis (P = 0.031), and Duke’s stage (P = 0.029) of CRC patients. Patients with higher HMGB1 expression had shorter overall survival time, whereas patients with lower level of HMGB1 had better survival. Multivariate analysis suggested that HMGB1 expression might be an independent prognostic indicator for the survival of patients with CRC. Disruption of endogenous HMGB1 protein through a siRNA knockdown technique was shown to suppress substantially the invasion ability of SW620 cells.

Conclusions

Our results suggest that HMGB1 protein is a valuable marker for progression of CRC patients. High HMGB1 expression is associated with poor overall survival in patients with CRC.

Keywords

HMGB1ProgressionPrognosisColorectal cancer

Copyright information

© Springer-Verlag 2009