Journal of Cancer Research and Clinical Oncology

, Volume 135, Issue 4, pp 507–514

Increased expressions of cannabinoid receptor-1 and transient receptor potential vanilloid-1 in human prostate carcinoma

  • Gabriella Czifra
  • Attila Varga
  • Katalin Nyeste
  • Rita Marincsák
  • Balázs I. Tóth
  • Ilona Kovács
  • László Kovács
  • Tamás Bíró
Original Paper

DOI: 10.1007/s00432-008-0482-3

Cite this article as:
Czifra, G., Varga, A., Nyeste, K. et al. J Cancer Res Clin Oncol (2009) 135: 507. doi:10.1007/s00432-008-0482-3

Abstract

Purpose

Recently, functional cannabinoid receptor-1 (CB1) and vanilloid receptor-1 (TRPV1) have been described in human prostate and prostate cancer-derived cell lines where the activation of the receptors resulted in inhibition of cellular growth. We, however, lack the description of the expression of these molecules in human prostate cancer (PCC) and in benign prostate hyperplasia (BPH).

Methods

Therefore, immunohistochemistry, Western blotting, and quantitative “real-time Q-PCR were performed to define the expressions of CB1 and TRPV1 in healthy and diseased prostate tissues.

Results

CB1 was identified in epithelial and smooth muscle cells types of the human prostate, whereas TRPV1 was exclusively localized to the mucosal cells. We also found that the expression of CB1 and TRPV1 (both at the protein and mRNA levels) were significantly up-regulated in PCC. However, while the increased expression of TRPV1 showed a proper correlation with increasing PCC tumor grades, such phenomenon was not observed with CB1. In addition, we also measured markedly elevated CB1 levels in BPH tissues whilst the expression of TRPV1 was not altered when compared to healthy control prostate.

Conclusions

Our findings strongly argue for that (1) the CB1 and TRPV1 molecules as well as their ligands may indeed possess a promising future role in the treatment of PCC; (2) TRPV1 may also serve as a prognostic factor in PCC; and (3) CB1 may act as a potential target molecule in the therapeutic management of BPH.

Keywords

Benign prostate hyperplasiaCannabinoid receptor-1 (CB1)Human prostate carcinomaTransient receptor potential vanilloid-1 (TRPV1)

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Gabriella Czifra
    • 1
  • Attila Varga
    • 2
  • Katalin Nyeste
    • 1
  • Rita Marincsák
    • 1
  • Balázs I. Tóth
    • 1
  • Ilona Kovács
    • 3
  • László Kovács
    • 1
  • Tamás Bíró
    • 1
  1. 1.Department of Physiology, Medical and Health Science Center, Research Center for Molecular MedicineUniversity of DebrecenDebrecenHungary
  2. 2.Department of Urology, Medical and Health Science Center, Research Center for Molecular MedicineUniversity of DebrecenDebrecenHungary
  3. 3.Department of PathologyKenézy HospitalDebrecenHungary