Original Paper

Journal of Cancer Research and Clinical Oncology

, Volume 134, Issue 12, pp 1363-1369

First online:

Microarray-based identification of CUB-domain containing protein 1 as a potential prognostic marker in conventional renal cell carcinoma

  • Yasuo AwakuraAffiliated withDepartment of Urology, Kyoto University Graduate School of Medicine
  • , Eijiro NakamuraAffiliated withDepartment of Urology, Kyoto University Graduate School of Medicine
  • , Takeshi TakahashiAffiliated withDepartment of Urology, Kyoto University Graduate School of Medicine
  • , Hirokazu KotaniAffiliated withLaboratory of Anatomic Pathology, Kyoto University Hospital
  • , Yoshiki MikamiAffiliated withLaboratory of Anatomic Pathology, Kyoto University Hospital
  • , Tadashi KadowakiAffiliated withDepartment of Genomic Drug Discovery Science, Kyoto University Graduate School of Pharmaceutical Sciences
  • , Akira MyoumotoAffiliated withNew Frontiers Research Laboratories, Toray Indutries Inc.
  • , Hideo AkiyamaAffiliated withNew Frontiers Research Laboratories, Toray Indutries Inc.
  • , Noriyuki ItoAffiliated withDepartment of Urology, Kyoto University Graduate School of Medicine
    • , Toshiyuki KamotoAffiliated withDepartment of Urology, Kyoto University Graduate School of Medicine
    • , Toshiaki ManabeAffiliated withLaboratory of Anatomic Pathology, Kyoto University Hospital
    • , Hitoshi NobumasaAffiliated withNew Frontiers Research Laboratories, Toray Indutries Inc.
    • , Gozoh TsujimotoAffiliated withDepartment of Genomic Drug Discovery Science, Kyoto University Graduate School of Pharmaceutical Sciences
    • , Osamu OgawaAffiliated withDepartment of Urology, Kyoto University Graduate School of Medicine Email author 

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Abstract

Purpose

Renal cell carcinoma (RCC) is characterized by a variable and unpredictable clinical course. Thus, accurate prediction of the prognosis is important in clinical settings. We conducted microarray-based study to identify a novel prognostic marker in conventional RCC.

Patients and methods

The present study included the patients surgically treated at Kyoto University Hospital. Gene expression profiling of 39 samples was carried out to select candidate prognostic markers. Quantitative real-time PCR of 65 samples confirmed the microarray experiment results. Finally, we evaluated the significance of potential markers at their protein expression level by immunohistochemically analyzing 230 conventional RCC patients.

Results

Using expression profiling analysis, we identified 14 candidate genes whose expression levels predicted unfavorable disease-specific survival. Next, we examined the expression levels of nine candidate genes by quantitative real-time PCR and selected CUB-domain containing protein 1 (CDCP1) for further immunohistochemical analysis. Positive staining for CDCP1 inversely correlated with disease-specific and recurrence-free survivals. In multivariate analysis including clinical/pathological factors, CDCP1 staining was a significant predictor of disease-specific and recurrence-free survivals.

Conclusions

We identified CDCP1 as a potential prognostic marker for conventional RCC. Further studies might be required to confirm the prognostic value of CDCP1 and to understand its function in RCC progression.

Keywords

CDCP1 Microarray Prognosis Renal cell carcinoma