Journal of Cancer Research and Clinical Oncology

, 134:1397

Novel treatment of ovarian cancer cell lines with Imatinib mesylate combined with Paclitaxel and Carboplatin leads to receptor-mediated antiproliferative effects

  • Christoph Mundhenke
  • Marion Tina Weigel
  • Klarissa Hanja Sturner
  • Frank Roesel
  • Ivo Meinhold-Heerlein
  • Dirk O. Bauerschlag
  • Christian Schem
  • Felix Hilpert
  • Walter Jonat
  • Nicolai Maass
Original Paper

DOI: 10.1007/s00432-008-0408-0

Cite this article as:
Mundhenke, C., Weigel, M.T., Sturner, K.H. et al. J Cancer Res Clin Oncol (2008) 134: 1397. doi:10.1007/s00432-008-0408-0
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Abstract

Purpose

Imatinib is a small molecule inhibiting the tyrosine kinases bcr-abl, c-kit, PDGFR-α and PDGFR-β. Investigations were performed to screen ovarian cancer cell lines and tumor samples for target receptor expression. Effects of Imatinib on cell proliferation and apoptosis induction were measured with and without additional cytotoxic agents.

Methods

Expression patterns of abl, c-kit, PDGFR-α and PDGFR-β (Imatinib targets) were studied in 5 cell lines and 111 tissue arrays by PCR and immunohistochemistry. Proliferation assays were performed with single agent Imatinib or combined with Paclitaxel and Carboplatin. Apoptosis was measured by DNA fragmentation.

Results

All cell lines expressed abl and PDGFR-β. C-kit was only expressed in 2/5 cell lines and PDGFR-α in 4/5. Imatinib reduced cell growth and lead to pro-apoptotic changes. Combination of Carboplatin, Paclitaxel and Imatinib showed synergistic activity.

Conclusions

Our results suggest that Imatinib may be useful for the specific treatment of ovarian cancer as an add-on to conventional chemotherapy.

Keywords

Ovarian cancerTargeted therapyIn vitroImatinib Mesylate

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Christoph Mundhenke
    • 1
  • Marion Tina Weigel
    • 1
  • Klarissa Hanja Sturner
    • 1
  • Frank Roesel
    • 1
  • Ivo Meinhold-Heerlein
    • 1
  • Dirk O. Bauerschlag
    • 1
  • Christian Schem
    • 1
  • Felix Hilpert
    • 1
  • Walter Jonat
    • 1
  • Nicolai Maass
    • 1
  1. 1.Department of Obstetrics and GynecologyCampus Kiel, University of KielKielGermany