Journal of Cancer Research and Clinical Oncology

, Volume 133, Issue 9, pp 673–678

Alcohol, smoking and human papillomavirus in laryngeal carcinoma: a Nordic prospective multicenter study

  • Walter J. Koskinen
  • Kjell Brøndbo
  • Hanna Mellin Dahlstrand
  • Tapio Luostarinen
  • Timo Hakulinen
  • Ilmo Leivo
  • Anco Molijn
  • Wim G. Quint
  • Tov Røysland
  • Eva Munck-Wikland
  • Antti A. Mäkitie
  • Ilmari Pyykkö
  • Joakim Dillner
  • Antti Vaheri
  • Leena-Maija Aaltonen
Original Paper

DOI: 10.1007/s00432-007-0219-8

Cite this article as:
Koskinen, W.J., Brøndbo, K., Mellin Dahlstrand, H. et al. J Cancer Res Clin Oncol (2007) 133: 673. doi:10.1007/s00432-007-0219-8

Abstract

Purpose

Human papillomavirus (HPV) has been linked to oropharyngeal carcinomas, but its role in laryngeal squamous cell carcinoma (LSCC) is not clear. A prospective multicenter study based on known tumor-cell percentage of fresh frozen carcinoma biopsies was established to determine the HPV prevalence. Moreover risk factors such as smoking, alcohol abuse, chronic laryngitis and gastroesophageal reflux disease (GERD) were evaluated

Methods

Fresh-frozen laryngeal cancer biopsies from 108 patients in Finland, Norway, and Sweden were investigated. Patients whose biopsy samples contained at least 20% tumor tissue (N = 69) entered the study. HPV DNA was determined with MY09/11 and GP5+/6+ nested PCR and SPF10 PCR hybridization assay. Patients were examined by an ENT specialist and an extensive questionnaire concerning risk factors was filled in.

Results

Only three patients (4.4%) harbored HPV DNA in their carcinoma sample. Heavy alcohol drinking was associated with an increased risk of death, advanced-stage disease, and younger age at diagnosis. Chronic laryngitis, GERD, and orogenital sex contacts were rare. Poor oral hygiene was not associated with survival, although it correlated with heavy drinking.

Conclusion

In our series HPV was not important in LSCC. Heavy drinking led to major mortality in LSCC and promoted early carcinogenesis.

Keywords

Chronic laryngitisGastroesophageal reflux diseaseLaryngeal cancerSquamous cell carcinoma

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Walter J. Koskinen
    • 1
    • 2
  • Kjell Brøndbo
    • 3
  • Hanna Mellin Dahlstrand
    • 4
  • Tapio Luostarinen
    • 5
  • Timo Hakulinen
    • 5
  • Ilmo Leivo
    • 6
  • Anco Molijn
    • 7
  • Wim G. Quint
    • 7
  • Tov Røysland
    • 3
  • Eva Munck-Wikland
    • 8
  • Antti A. Mäkitie
    • 2
  • Ilmari Pyykkö
    • 9
  • Joakim Dillner
    • 10
  • Antti Vaheri
    • 1
  • Leena-Maija Aaltonen
    • 2
  1. 1.Department of Virology, Haartman InstituteUniversity of HelsinkiHelsinkiFinland
  2. 2.Department of Otorhinolaryngology—Head and Neck SurgeryHelsinki University Central HospitalHelsinkiFinland
  3. 3.Department of OtorhinolaryngologyRikshospitaletOsloNorway
  4. 4.Department of OncologyKarolinska University HospitalStockholmSweden
  5. 5.Finnish Cancer RegistryHelsinkiFinland
  6. 6.Department of PathologyUniversity of Helsinki and Haartman Laboratory Diagnostics, Helsinki University Central HospitalHelsinkiFinland
  7. 7.Delft Diagnostic LaboratoryVoorburgThe Netherlands
  8. 8.Department of Otorhinolaryngology—Head and Neck SurgeryKarolinska University HospitalStockholmSweden
  9. 9.Department of OtorhinolaryngologyTampere University HospitalTampereFinland
  10. 10.Department of Medical MicrobiologyMalmö University HospitalMalmöSweden