Journal of Cancer Research and Clinical Oncology

, Volume 133, Issue 2, pp 83–92

Dominant-negative E-cadherin inhibits the invasiveness of inflammatory breast cancer cells in vitro

  • Hui-Ming Dong
  • Gang Liu
  • Yi-Feng Hou
  • Jiong Wu
  • Jin-Song Lu
  • Jian-Min Luo
  • Zhen-Zhou Shen
  • Zhi-Ming Shao
Original Paper

DOI: 10.1007/s00432-006-0140-6

Cite this article as:
Dong, HM., Liu, G., Hou, YF. et al. J Cancer Res Clin Oncol (2007) 133: 83. doi:10.1007/s00432-006-0140-6

Abstract

E-cadherin is a transmembrane glycoprotein which mediates epithelial cell-to-cell adhesion function as a tumor suppressor and frequently loss of expression in a wide spectrum of human cancer. However, recent studies demonstrated that E-cadherin was always over-expressed in inflammatory breast cancer (IBC) specimen and cell lines, which is a clinical extreme malignancy of breast cancer. It is hypothesized that the gain and not the loss of the E-cadherin axis contributes to the IBC unique phenotype. To test this assumption, we generated dominant negative mutant E-cadherin high-expression inflammatory breast cancer cells by introduced dominant negative mutant E-cadherin (H-2kd-E-cad) cDNA into human IBC SUM149 cells. Our studies demonstrated that the ability of invasion of SUM149 cells was significantly inhibited by H-2kd-E-cad via down-regulation of MMP-1 and MMP-9 expression. The underlying signal pathway of MAPK phosphorylated Erk 1/2(P44/42) in H-2kd-E-cad-transfected SUM149 cells was significantly down-regulated compared to parental and mock contrast. Our studies provided further functional evidence as the gain of E-cadherin expression dedicated to the IBC malignant phenotype and the blockage of MAPK/Erk activation maybe a promising therapeutic target.

Keywords

E-cadherinInflammatory breast cancerMMPMAPK

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Hui-Ming Dong
    • 1
  • Gang Liu
    • 1
  • Yi-Feng Hou
    • 1
  • Jiong Wu
    • 1
  • Jin-Song Lu
    • 1
  • Jian-Min Luo
    • 1
  • Zhen-Zhou Shen
    • 1
  • Zhi-Ming Shao
    • 1
  1. 1.Department of Breast SurgeryBreast Cancer Institute, Cancer Hospital/Cancer Institute, Fudan UniversityShanghaiPeople’s Republic of China