Gastric and intestinal phenotypic cell marker expressions in gastric differentiated-type carcinomas: association with E-cadherin expression and chromosomal changes

  • Koji Morohara
  • Yusuke Tajima
  • Kentaro Nakao
  • Nobukazu Nishino
  • Shigeo Aoki
  • Masanori Kato
  • Masaaki Sakamoto
  • Kimiyasu Yamazaki
  • Tsutomu Kaetsu
  • Satoshi Suzuki
  • Akira Tsunoda
  • Tetsuhiko Tachikawa
  • Mitsuo Kusano
Original Paper

DOI: 10.1007/s00432-005-0062-8

Cite this article as:
Morohara, K., Tajima, Y., Nakao, K. et al. J Cancer Res Clin Oncol (2006) 132: 363. doi:10.1007/s00432-005-0062-8

Abstracts

Gastric and intestinal phenotypic cell markers are widely expressed in gastric carcinomas, irrespective of their histological type. In the present study, the relations between the phenotypic marker expression of the tumour, histological findings, expression of cell adhesion molecules, and the chromosomal changes in gastric differentiated-type carcinomas were examined. The phenotypic marker expression of the tumour was determined by the combination of the expression of the human gastric mucin (HGM), MUC6, MUC2 and CD10, and was evaluated in comparison with the expression of cell adhesion molecules, such as E-cadherin and β-catenin, and chromosomal changes by comparative genomic hybridization (CGH) in 34 gastric differentiated-type carcinomas. Tumours were classified into the gastric- (G-), gastric and intestinal mixed- (GI-), intestinal- (I-), or unclassified- (UC-) phenotype according to the immunopositivity of staining for HGM, MUC6, MUC2, and CD10. G-phenotype tumours were significantly associated with a higher incidence of differentiated-type tumours mixed with undifferentiated-type component, compared with GI- and I-phenotype tumours (88.9 vs 33.3%, P=0.0498 and 88.9 vs 42.9%, P=0.0397; respectively). HGM-positive tumours were significantly associated with a higher incidence of tumours with abnormal expression of E-cadherin, compared with HGM-negative tumours (66.7 vs 21.1%, P=0.0135). GI-phenotype tumours were significantly associated with a higher incidence of tumours with abnormal expression of E-cadherin, compared with I-phenotype tumours (77.8 vs 21.4%, P=0.0131). HGM-negative tumours were significantly associated with higher frequencies of the gains of 19q13.2 and 19q13.3, compared with HGM-positive tumours (57.9 vs 20.0%, P=0.0382 and 63.2 vs 13.3%, P=0.0051; respectively). MUC6-positive tumours were significantly associated with higher frequencies of the gains of 20q13.2, compared with MUC6-negative tumours (71.4 vs 30.0%, P=0.0349). MUC2-positive tumours were significantly associated with the gain of 19p13.3, compared with MUC2-negative tumours (41.2 vs 5.9%, P=0.0391). I-phenotype tumours were significantly associated with higher frequencies of gains of 5p15.2 and 13q33-34, compared with G-phenotype tumours (66.7 vs 0%, P=0.0481, each) and also associated with higher frequencies of gain of 7p21, compared with GI-phenotype tumours (66.7 vs 0%, P=0.0481). Our present results show that gastric differentiated-type carcinomas have different characteristics according to the phenotypic marker expression of the tumour in terms of histological findings, E-cadherin expression and pattern of chromosomal changes.

Keywords

Gastric carcinoma Human gastric mucin MUC6 MUC2 CD10 E-cadherin β-catenin Comparative genomic hybridization 

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Koji Morohara
    • 1
  • Yusuke Tajima
    • 1
  • Kentaro Nakao
    • 1
  • Nobukazu Nishino
    • 1
  • Shigeo Aoki
    • 1
  • Masanori Kato
    • 1
  • Masaaki Sakamoto
    • 1
  • Kimiyasu Yamazaki
    • 1
  • Tsutomu Kaetsu
    • 1
  • Satoshi Suzuki
    • 1
  • Akira Tsunoda
    • 1
  • Tetsuhiko Tachikawa
    • 2
  • Mitsuo Kusano
    • 1
  1. 1.Department of Surgery, Division of General & Gastroenterological SurgeryShowa University, School of MedicineTokyoJapan
  2. 2.Department of Oral PathologyShowa University, School of DentistryTokyoJapan