Original Paper

Journal of Cancer Research and Clinical Oncology

, Volume 132, Issue 2, pp 92-98

First online:

Proteomic analysis on metastasis-associated proteins of human hepatocellular carcinoma tissues

  • Hai-Yan SongAffiliated withLiver Cancer Institute, Zhongshan Hospital, Fudan University
  • , Yin-Kun LiuAffiliated withLiver Cancer Institute, Zhongshan Hospital, Fudan University Email author 
  • , Ju-Tao FengAffiliated withLiver Cancer Institute, Zhongshan Hospital, Fudan University
  • , Jie-Feng CuiAffiliated withLiver Cancer Institute, Zhongshan Hospital, Fudan University
  • , Zhi DaiAffiliated withLiver Cancer Institute, Zhongshan Hospital, Fudan University
  • , Li-Jun ZhangAffiliated withDepartment of Molecular Genetics, Shanghai Medical College, Fudan University
  • , Jiu-Xian FengAffiliated withChest Hospital
  • , Hua-Li ShenAffiliated withDepartment of Chemistry, Fudan University
  • , Zhao-You TangAffiliated withLiver Cancer Institute, Zhongshan Hospital, Fudan University

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Purpose: A comparative proteomic approach was used to identify and analyze proteins related to metastasis of hepatocellular carcinoma (HCC). Methods: Proteins extracted from 12 HCC tissue specimens (six with metastases and six without) were separated by two-dimensional gel electrophoresis (2-DE). The protein spots exhibiting statistical alternations between the two groups through computerized image analysis were then identified by mass spectrometry. In addition immunohistochemistry (IHC), Western blotting and RT-PCR were performed to verify the expression of certain candidate proteins. Results: 16 proteins including HSP27, S100A11, CK18 were annotated by mass spectrometry, relevant to chaperone function, cell mobility, cytoskeletal architecture, respectively. Most were previously unconnected with metastasis of HCC. Of these HSP27 was found overexpressed consistently in 2-DE patterns of all metastatic HCC tissues compared with nonmetastatic ones. IHC and Western blotting of HCC tissues confirmed this difference while RT-PCR did not. Conclusion: There are various proteins joined together in HCC metastasis. The overexpression of HSP27 may serve as a biomarker for early detection and therapeutic targets unique to the metastatic phenotype of HCC.

Keywords

Human hepatocellular carcinoma Metastasis Two-dimensional gel electrophoresis Heat shock protein 27