Medical Microbiology and Immunology

, Volume 189, Issue 4, pp 225–229

Antibodies to Lassa virus Z protein and nucleoprotein co-occur in human sera from Lassa fever endemic regions

  • Stephan Günther
  • Olaf Kühle
  • Daniela Rehder
  • Georgina N. Odaibo
  • David O. Olaleye
  • Petra Emmerich
  • Jan ter Meulen
  • Herbert Schmitz
Original Investigation

DOI: 10.1007/s004300100061

Cite this article as:
Günther, S., Kühle, O., Rehder, D. et al. Med Microbiol Immunol (2001) 189: 225. doi:10.1007/s004300100061

Abstract

It is not known whether the small 11-kDa Z protein of Lassa virus is immunogenic during human Lassa virus infection. To obtain evidence for the existence of an antibody response and to test the suitability of these antibodies for serosurveys, sera from Lassa fever endemic regions (Guinea and Nigeria, n=75) were tested for co-reactivity to Z protein and nucleoprotein (NP). Sera from a non-endemic region (Uganda, n=50) served as a specificity control. Z protein and NP were expressed in Escherichia coli, affinity-purified, and used as antigen in Western blot. Indirect immunofluorescence (IIF) with Lassa virus-infected cells was performed for comparison. Due to high unspecific reactivity of the African sera, Western blot testing was performed with a 1:1,000 serum dilution. Under these conditions, none of the control sera but 12% of the sera from endemic regions co-reacted with both Z protein and NP. Reactivity to Z protein was significantly associated with NP reactivity (P<10–6). NP and Z protein-specific antibodies were co-detected in 33% of the IIF-positive sera and in 5% of the IIF-negative sera (P=0.001). These data provide evidence for appearance of antibodies to Z protein and NP following Lassa virus infection. A recombinant blot for detection of both antibody specificities seems to be specific but less sensitive than IIF.

Lassa virus Antibody response Immunofluorescence Western blot

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Stephan Günther
    • 1
  • Olaf Kühle
    • 1
  • Daniela Rehder
    • 1
  • Georgina N. Odaibo
    • 2
  • David O. Olaleye
    • 2
  • Petra Emmerich
    • 1
  • Jan ter Meulen
    • 1
  • Herbert Schmitz
    • 1
  1. 1.Bernhard-Nocht-Institut für Tropenmedizin, Bernhard-Nocht-Strasse 74, 20359 Hamburg, GermanyGermany
  2. 2.Department of Virology, College of Medicine, University of Ibadan, Ibadan, NigeriaNigeria
  3. 3.Projet de Recherche sur les Fièvres Hémorragiques en Guinée, Conakry, Republic of GuineaRepublic of Guinea
  4. 4.EPICENTRE (Groupe Europeéne d'Expertise en Epidemiologie), Paris, FranceFrance
  5. 5.Present address: Institute of Virology, Philipps University, 35037 Marburg, GermanyGermany