Medical Microbiology and Immunology

, Volume 186, Issue 4, pp 189–194

Persistent parvovirus B19 infections in immunocompromised children

  • Gisela Flunker
  • Angelika Peters
  • Siegfried Wiersbitzky
  • Susanne Modrow
  • W. Seidel
ORIGINAL INVESTIGATION

DOI: 10.1007/s004300050063

Cite this article as:
Flunker, G., Peters, A., Wiersbitzky, S. et al. Med Microbiol Immunol (1998) 186: 189. doi:10.1007/s004300050063

Abstract

Immunocompromised patients have been shown to suffer from prolonged viral infections often without detectable immune response. Here chronic infections with low virus levels can be frequently observed. In these patients viral DNA can be detected over long periods by polymerase chain reaction (PCR). In this study parvovirus B19 presence was assessed by PCR, immunoblot and enzyme-linked immunosorbent assay in sera from children with mainly oncological and hematological diseases. In 45% of sera B19 DNA was observed. Of the children 25% had IgG antibodies to viral protein 1 and 2 (VP1/2) and 15% to nonstructural protein 1 (NS1). In 6% of children IgM antibodies to VP1/2 were detected. These results indicate that the number of children with immune response to B19 proteins is distinctly lower than the number of children with B19 DNA. Transfusions of blood products might have been a possible route for B19 infection. Establishment and maintenance of a persistent parvovirus B19 infection with or without immune response are enhanced in the analyzed immunocompromised children in comparison with immunocompetent children. A persistence of B19 DNA was demonstrated up to 10 months in patients sera.

Key words Parvovirus B19 persistencePatients with immune defectPCRNonstructural proteinImmune response

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Gisela Flunker
    • 1
  • Angelika Peters
    • 2
  • Siegfried Wiersbitzky
    • 2
  • Susanne Modrow
    • 3
  • W. Seidel
    • 1
  1. 1.Institut für Medizinische Mikrobiologie, Abteilung Virologie, Universität Greifswald, Martin-Luther-Strasse 6, D-17487 Greifswald, Germany, Tel.: 49-3834-865570; Fax: 49-3834-865568; e-mail: seidel@rz.uni-greifswald.deDE
  2. 2.Zentrum für Kinder- und Jugendmedizin, Klinik und Poliklinik für Kindermedizin, Universität Greifswald, Soldtmannstrasse 15, D-17487 Greifswald, GermanyDE
  3. 3.Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Franz-Josef-Strauss-Allee 11, D-93053 Regensburg, GermanyDE