Medical Microbiology and Immunology

, Volume 185, Issue 1, pp 19–25

Expansion of T cells negative for CD28 expression in hiv infection. Relation to activation markers and cell adhesion molecules, and correlation with prognostic markers

  • R. Kämmerer
  • A. Iten
  • P. C. Frei
  • P. Bürgisser
ORIGINAL INVESTIGATION

DOI: 10.1007/s004300050010

Cite this article as:
Kämmerer, R., Iten, A., Frei, P. et al. Med Microbol Immunol (1996) 185: 19. doi:10.1007/s004300050010

Abstract

  CD28 is a transmembrane glycoprotein that provides T cells with an essential co-stimulatory signal during antigen presentation. Using flow cytometry, we document here an expansion of CD28 T cells in HIV infection. Whereas the percentage of CD4+CD28+ T cells among total lymphocytes was decreased, a small increase of the percentage of CD4+CD28 T cells was observed. In the CD8+ subset, there was a marked expansion of CD8+CD28 T cells. An increased percentage of CD8+ T cells positive for HLA-DR was found in both CD28+ and CD28 cells. Results were similar for CD38 expression. HIV infection was also distinguished by a shift from LFA-1lowCD28low to LFA-1highCD28high and LFA-1high-CD28neg expression pattern on CD8+ T cells. Negative correlations were found between percentage and absolute number of CD8+CD28+ T cells and several serum parameters usually associated with poor prognosis (IgA, IgE, β2-microglobulin and HIV-1 p24 antigen). Thus, HIV infection is characterized by a marked expansion of CD28 T cells with an abnormal expression of activation markers and cell adhesion molecules. In addition, CD8+CD28+, but not CD8+CD28 or total CD8+ T cell numbers, correlated with the levels of established serological markers of disease severity or progression and may, therefore, have predictive value.

Key words  CD28Cell adhesion moleculesCell activation markersSerum prognostic markersHuman immunodeficiency virus type 1

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • R. Kämmerer
    • 1
  • A. Iten
    • 3
  • P. C. Frei
    • 2
  • P. Bürgisser
    • 2
  1. 1.Institute for Medical Microbiology, Medizinische Universität zu Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, GermanyDE
  2. 2.Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, CH-1011 Lausanne, SwitzerlandCH
  3. 3.Division of Infectious Diseases, Centre Hospitalier Universitaire Vaudois, CH-1011 Lausanne, SwitzerlandCH