Medical Microbiology and Immunology

, Volume 189, Issue 2, pp 75–83

Inducing protective antibodies against ring-infected erythrocyte surface peptide antigen of Plasmodium falciparum using immunostimulating complex (ISCOMs) delivery

  • Neeraj Chopra
  • Shukla Biswas
  • Beena Thomas
  • Leenu Sabhnani
  • D. N. Rao
ORIGINAL INVESTIGATION

DOI: 10.1007/s004300000042

Cite this article as:
Chopra, N., Biswas, S., Thomas, B. et al. Med Microbiol Immunol (2000) 189: 75. doi:10.1007/s004300000042

Abstract

In the present study, synthetic peptides (EENVEHDA)2 [(oc)2] and (DDEHVEEPTVA)2 [(un)2] of ring-infected erythrocyte surface antigen (RESA) of Plasmodium falciparum were linked with palmitic acid and entrapped in immunostimulating complexes (ISCOMs). The immunogenicity of the peptide(s) and mixture of peptides were studied in mice with different genetic background. Peptide(s) entrapped in ISCOMs using a low-dose immunization strategy generated high-titer as well as high-affinity antibodies. Interestingly, no genetic restriction of the immune response was observed in any of the strains studied. The IgG subclass pattern with the peptide(s) showed predominately IgG2a/2b isotypes, while with the mixed peptide formulation, (un)2-specific IgG isotype pattern showed induction of both IgG1 and IgG2a/2b isotypes. These cytophilic antibodies inhibited the ring as well as schizont stage and total parasite growth during in vitro merozoite reinvasion inhibition study. In the mixed peptide preparation, the same pattern of immune response was achieved as that of individual peptide(s) using ISCOMs delivery. Therefore, the entrapment of otherwise poorly immunogenic synthetic peptides in ISCOMs resulted in increased immunogenicity followed by strong secondary response and can be adopted for developing subunit immunogen formulation against malarial parasite.

Key words Ring-infected erythrocyte surface antigen peptides Immunostimulating complexes Inbred mice Protective antibodies 

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Neeraj Chopra
    • 1
  • Shukla Biswas
    • 2
  • Beena Thomas
    • 1
  • Leenu Sabhnani
    • 1
  • D. N. Rao
    • 1
  1. 1.Department of Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110 029, India e-mail: dnrao@medinst.ernet.in Tel.: +91-11-6593545; Fax: +91-11-6862663IN
  2. 2.Malaria Research Centre, 22 Sham Nath Marg, Delhi-110 054, IndiaIN

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